Environmental sampling has become a commonly accepted diagnostic technique for means of identifying breaks in biosecurity. However, environmental samples have yet to be validated reverse transcriptase real-time PCR (qRT-PCR) analysis and there is no standardization sample processing. Therefore, the objective this project was evaluate different types samples, whether processing prior qRT-PCR wou...

Purpose: The aims of this study were to investigate the clinical value Rab27a as a urinary biomarker, and its efficiency in prediction bladder cancer grade.Materials Methods: expression urine samples patients with cancer, cell line (T-24), tissue was estimated via quantitative reverse transcription polymerase chain reaction (qRT-PCR). level investigated according sex, age, histological grade qR...

results comparative analysis of predicted amino acids in the phylogenetic tree showed high similarity between c. glabrata and s cerevisiae. besides, c. glabrata demonstrated the capability to grow in glucose levels as low as 0.01% in the spot dilution assay. in qrt-pcr analysis, differential expressions were observed in selected genes when c. glabrata was subjected to different glucose concentr...

Background: IL-4 is a cytokine that induces differentiation of naive helper T cells into Th2 cells. Once activated by IL-4, Th2 cells subsequently produce additional IL-4. Objective: To examine the effect of IL-4 on IL-17 production and its effect in Collagen- Induced Arthritis (CIA) mice. Method: In this study, a chicken collagen-II-induced experimental arthritis (CIA) model was used in DBA/1 ...

For design flood estimation in ungauged catchments, Regional Flood Frequency Analysis (RFFA) is commonly used. Most of the RFFA methods are primarily based on linear modelling approaches, which do not account for inherent nonlinearity rainfall-runoff processes. Using data from 114 catchments Victoria, Australia, this study employs Generalised Additive Model (GAM) and compares results with metho...

Abstract Background Circular RNAs (circRNAs) have been reported to be important regulators of the development and progression various carcinomas. However, role circRNA_100290 in gastric cancer (GC) is still unclear. This study aimed investigate GC invasion metastasis possible underlying mechanism. Methods The expression cells tissues was examined using quantitative real-time polymerase chain re...