Hereditary hemochromatosis (HHC) represents an autosomal recessive disease in which increased iron absorption causes iron overload and irreversible tissue damage. The recently detected association between two point mutations in the HFE gene on chromosome 6p and HHC has made it possible to screen for the disease before the onset of irreversible tissue damage. Conventional genetic testing is base...

Genotypes of Iranian Zel sheep for Calpastatin(CAST) locus were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) methods and for Calpain(CAPN) locus by PCR-SSCP. Blood samples were collected from 200 purebred Zel sheep of Zel Breeding Station located in Golestan province i...

In the early 1990’s two genes, BRCAl and BRCA2 were discovered that predispose to both breast and ovarian cancers. Although by current estimates only 5–10% of these cancers are hereditary, the identification of susceptibility genes allows the application of oncogenetics as an alternative method of early detection. Indeed several mutations have been identified in these two genes some of which ch...

Tkáãiková a. , M. R. , Bhide I . Mikula: Asymmetric PCR-SSCP: a Useful Tool for Detection of OLA-DRB1(MHC class II) Gene Polymorphism in Slovak Improved Valachian Sheep. Acta Vet. Brno 2005, 74: 275-278. Detection of OLA-DBR1 (exon 2) gene polymorphism is presented in the paper. Rapid and inexpensive polymorphism detection method, namely, single stranded conformation polymorphism (SSCP) was ass...

Our purpose was to search for mutations in the apolipoprotein E (apoE) gene and to evaluate the role of apoE polymorphisms in the occurrence of autosomal dominant retinitis pigmentosa (ADRP). The ApoE gene coding sequence was analyzed in 51 unrelated patients affected with ADRP. A screening for mutations by SSCP and an analysis of the apoE polymorphisms were performed using PCR and restriction ...

radioactive iodine therapy is commonly used as an adjuvant therapy in follicular and papillary thyroid carcinoma and also in the treatment of graves’ disease. the basis of which is the accumulation of radioactive iodine by the sodium-iodide symporter (nis) in the thyroid gland. expression of nis by extrathyroidal tissues including islets of pancreas has been reported. radioactive iodine uptake ...

We consider the problem of finding the localization of radioactive source by using data from a digital camera. In other words, the camera could help us to detect the direction of radioactive rays radiation. Therefore, the outcome could be used to command a robot to move toward the true direction to achieve the source. The process of camera data is performed by using image processing and computa...

Enzyme is a dynamic entity with diverse time scales, ranging from picoseconds to seconds or even longer. Here we develop a rate theory for enzyme catalysis that includes conformational dynamics as cycling on a two-dimensional (2D) reaction free energy surface involving an intrinsic reaction coordinate (X) and an enzyme conformational coordinate (Q). The validity of Michaelis-Menten (MM) equatio...