SUMMARY Q-Gene is an application for the processing of quantitative real-time RT-PCR data. It offers the user the possibility to freely choose between two principally different procedures to calculate normalized gene expressions as either means of Normalized Expressions or Mean Normalized Expressions. In this contribution it will be shown that the calculation of Mean Normalized Expressions has ...

ROOM (Real-Time Object-Oriented Modeling) is an architecture description language widely used in the telecommunications industry to develop embedded software. The concepts of ROOM have been incorporated into the CASE tool Rational Rose Real-Time (RoseRT) in the form of a UML profile, commonly called UML-RT. However, UMLRT itself does not provide any support for performing realtime scheduling an...

The MPI/RT standard is the product of the work of many people working in an open community standards group over a period of six plus years. The purpose of this archival publication is to preserve the significant knowledge and experience that was developed in real-time message passing systems as a consequence of the R&D effort as well as in the specification of the standard. Interestingly, sever...

The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products. Use of appropriate chemistr...

Even though real-time systems have the stringent constraint of completing tasks before their deadlines, many existing real-time operating systems do not implement fault tolerance capabilities. In this paper we summarize fault tolerant real-time scheduling policy for dynamic tasks with ready times and deadlines. Our focus in this paper is the implementation, which includes fault-tolerant schedul...

Molecular methods are being increasingly applied to detect, quantify and study microbial populations in food or during food processes. Among these methods, PCR-based techniques have been the subject of considerable focus and ISO guidelines have been established for the detection of food-borne pathogens. More particularly, real-time quantitative PCR (qPCR) is considered as a method of choice for...