Allergies affect almost 25% of the population in industrialized countries. Alternaria alternata is known to be a significant source of aeroallergens and sensitization to this mold is a risk factor for the development of wheezing, asthma, and atopic dermatitis. Diagnosis and treatment of allergies requires the production of large amounts of pure and well defined protein. Yarrowia lipolytica, a n...

PURPOSE Divergent results on the IgE reactivity of dog-allergic subjects to Can f 4 have been reported. The aim of this study was to evaluate the significance of Can f 4 in dog allergy and to develop an immunochemical method for measuring Can f 4 content in environmental samples. METHODS We purified the natural dog allergen Can f 4 from a dog dander extract by monoclonal antibody-based affini...

Dermatophagoides farinae, as one species of house dust mites, was known to be rich in IgE-reactive components that contribute to allergy in China and worldwide. This study aims to characterize a novel allergen, the D. Farinae-derived Der f 25. In this study, total RNA was extracted from Dermatophagoides farinae and the full-length of Der f 25 gene was amplified by RT-PCR with the primers design...

Immunotherapy for allergic diseases represents an important but largely unmet medical need. Conventional immunotherapy suffers from several breakdowns related to the quality of the extracts used, the risk of inducing anaphylactic reactions, and the extremely long treatment time. Many of the problems associated with using natural allergenic products for allergy diagnosis and treatment can be ove...

from either grass or tree pollens, insect venoms, cat dander as well as house dust mites [2–13] ( fig. 1 ). Such fusion proteins can then be expressed in Escherichia coli , yeasts (e.g. Saccharomyces cerevisiae or Pichia pastoris ) and plant or mammalian host cell systems prior to purification and refolding. One advantage, obviously, is to produce the multiallergen drug substance as a single mo...

Background: Allergy is a clinical disorder affecting the human population with wide geographical distribution. Platanus orientalis (P. orientalis) trees are planted in many countries and their pollen causes allergic reactions. Cyclophilin has recently been identified as one of the most important allergens of P. orientalis pollen. We aimed to clone and purify this allergen in Escherichia coli...