Objective(s): Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analy...

Droplet-based microfluidic system for biogenic paramagnetic nanoparticle synthesis is reported in this study. The engineered recombinant Escherichia coli (E. coli), which expressed heavy metal-binding proteins, were injected to droplets in the microchannel together with iron and manganese solution. Homogeneous droplets were generated and went through a serpentine channel to mix the three compon...

The production of secreted recombinant proteins from E. coli is pivotal to the biotechnological industry because it reduces the cost of downstream processing. Proteins destined for secretion contain an N-terminal signal peptide that is cleaved by secretion machinery in the plasma membrane. The resulting protein is released in an active mature form. In this study, Bacillus subtilis chitosanase (...

This study on the structure-activity relationship of polymyxin B, a cyclic peptide antibiotic, used sixteen synthetic polymyxin B(3) analogs including alanine scanning analogs to elucidate the contribution of the side chains to antimicrobial activity and lipopolysaccharide (LPS) binding. Of these analogs, [Ala(5)]-polymyxin B(3) showed greatly reduced antimicrobial activity against Escherichia ...

Functional recombinant bovine β-lactoglobulin has been produced by expression in E. coli using an engineered protein gene and purified to homogeneity by applying a new protocol. Mutations L1A/I2S introduced into the protein sequence greatly facilitate in vivo cleavage of the N-terminal methionine, allowing correctly folded and soluble protein suitable for biochemical, biophysical and structural...

Protein production for structural and biophysical studies, functional assays, biomarkers, mechanistic studies in vitro and in vivo, but also for therapeutic applications in pharma, biotech and academia has evolved into a mature discipline in recent years. Due to the increased emphasis on biopharmaceuticals, the growing demand for proteins used for structural and biophysical studies, the impact ...

background and objectives: enterohemorrhagic escherichia coli (ehec) o157:h7 has been recognized as a major food borne pathogen responsible for frequent hemorrhagic colitis and hemolytic uremic syndrome in humans. cattle are important reservoirs of e. coli o157:h7, in which the organism colonizes the intestinal tract and is shed in the feces. objective: vaccination of cattle has significant pot...

This study aimed to facilitate efficient arsenic detoxification by inducing the microbial methylation of inorganic arsenic. Recombinant Escherichia coli strains K31 was transformed overexpress methyltransferase, and evaluated using enzyme extracted from strain. To ensure continuous reactions maintaining enzymatic activity, immobilized in microcapsules (MC) catalyze The total yield methylated or...