Pegylated interferon alpha (PEG-IFN-alpha) is a formulation of recombinant human interferon conjugated with polyethylene-glycol. This preparation has a long half-life (compared with conventional IFN), which allows for once-weekly injection. Elimination half-life of PEG-IFN-alpha 2b is 22-60 hours (average, 35-40 hours). We report the development of a local necrotizing reaction complicating subc...

human interferon β (inf-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. in this study an expression vector was designed and constructed for expression of human inf-β-1b either in shake flasks and bench top bioreactor. the designed vector was constructed based upon pet-25b(+) with t7 promoter. recombinant human beta interferon ...

PURPOSE To study the presence of muscarinic and alpha- and beta-adrenergic receptors in a normal human conjunctival epithelial (IOBA-NHC) cell line. METHODS Neurotransmitter receptors were determined in IOBA-NHC cells by flow cytometry, immunofluorescence, and Western blot analysis. Antibodies to M(1)-, M(2)-, and M(3)-muscarinic and to alpha(1A)-, alpha(1B)-, alpha(1D)-, alpha(2A)-, alpha(2B...

To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-alpha 2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-alpha 2-(1-110) and HuIFN-alpha 2-(111-153). After reduction with 2-mercaptoethanol and separation of the two major fragments on NaDodSO4/polyacrylamide g...

BACKGROUND Two formulations of Pegylated interferon (Peg-IFN) are on the market for treatment of chronic hepatitis C virus (HCV) infection. The purpose of this meta-analysis was to assess the efficacy of Peg-IFN α-2a versus Peg-IFN α-2b in combination with ribavirin in anti-human immunodeficiency virus (HIV)-negative patients with genotype 1 chronic HCV infection. METHODS The following criter...

With little improvement in the poor prognosis for humans with high-grade glioma brain tumors, alternative therapeutic strategies are needed. As such, selective replication-competent oncolytic viruses may be useful as a potential treatment modality. Here we test the hypothesis that defects in the interferon (IFN) pathway could be exploited to enhance the selective oncolytic profile of vesicular ...

Sir, (WellferonB , Glaxo-Wellcome), natural leukocyte (CilferonB , Janssen-Cilag) and raIFN (Intron-AB , Schering-Plough), 3 It is well known that prolonged administration of recombinant alpha interferon (raIFN) in several viral and malignant disMU intramuscularly, daily or on alternate days depending on the clinical outcome, evaluated as in our former paper (4). eases can be followed by loss o...

We examined the association between IL28B single-nucleotide polymorphism rs12979860, hepatitis C virus (HCV) kinetic, and pegylated interferon alpha-2a pharmacodynamic parameters in HIV/HCV-coinfected patients from South America. Twenty-six subjects received pegylated interferon alpha-2a + ribavirin. Serum HCV-RNA and interferon concentrations were measured frequently during the first 12 weeks ...