Large-scale cDNA-sequencing projects require an efficient strategy for mass sequencing. Here we describe a method for sequencing pooled cDNA clones using a combination of transposon insertion and Gateway technology. Our method reduces the number of shotgun clones that are unsuitable for reconstruction of cDNA sequences, and has the advantage of reducing the total costs of the sequencing project.

Here, we report the draft genome sequence of Desulfotomaculum hydrothermale, a sulfate-reducing, spore-forming bacterium isolated from a Tunisian hot spring. The genome is composed of 2.7 Mb, with a G+C content of 49.48%, and it contains 2,643 protein-coding sequences.

Xanthomonas fragariae is a worldwide-spread plant bacterial disease causing angular leaf spots, thus reducing the yield of production for strawberry fruits. Three isolates with various geographic and time origins were sequenced with long-read technology (PacBio) to generate finished genome sequences of virulent strains and observe the variability in their contents.

Natural attenuation of the environmental contaminant perchlorate is a cost-effective alternative to current removal methods. The success of natural perchlorate remediation is dependent on the presence and activity of dissimilatory (per)chlorate-reducing bacteria (DPRB) within a target site. To detect DPRB in the environment, two degenerate primer sets targeting the chlorite dismutase (cld) gene...

High-fi eld imaging of the GI tract at 3 T can be very attractive in terms of signal to noise ratio (SNR) when compared with 1.5 T imaging. However, several issues need to be addressed and a direct transition of sequences used at 1.5–3 T is not possible. The main constraints in 3 T imaging are related to changes in tissue T1 and T2 relaxation parameters, susceptibility artifacts, changes in eff...

Genetic diversity and community structure of sulfate-reducing bacteria was investigated down the 12 meter water column of Siders Pond, a meromictic salt pond in Falmouth, MA. The physical and chemical gradients down this water column were also investigated, as they provide distinct niches for the microorganisms of Siders Pond. Microbial DNA was extracted from water samples at 12 depths. The dis...

crude glycerol (cg) is an abundantly available and cheap by-product from biodiesel production. value-added applications for cg are highly wanted by industry and several processes such as the use of cg for enhanced oil recovery have been proposed. the aim of this study was to evaluate the sulfide production of sulfate-reducing bacteria (srb) indigenous to oil reservoirs using cg as substrate. th...

background: the approaches chosen for control of outbreaks of infectious diseases in aquatic farming industry include improvement of environmental conditions, stocking of specific pathogen free (spf) brood stockings, and application of vaccines and immunostimulants. despite numerous studies on the effects of ergosan on immune system of aquatic animals, there is no data available on antioxidant ...

Stimulating microbial reduction of soluble U(VI) to insoluble U(IV) shows promise as a strategy for immobilizing uranium in uranium-contaminated subsurface environments. In order to learn more about which microorganisms might be involved in U(VI) reduction in situ, the changes in the microbial community when U(VI) reduction was stimulated with the addition of acetate were monitored in sediments...

We compared the sensitivity of standard single-shot 2D echo planar imaging (EPI) to three advanced EPI sequences, i.e., 2D multi-echo EPI, 3D high resolution EPI and 3D dual-echo fast EPI in fixed effect and random effects group level fMRI analyses at 3T. The study focused on how well the variance reduction in fixed effect analyses achieved by advanced EPI sequences translates into increased se...