نتایج جستجو برای: reflects his great genius
تعداد نتایج: 440027 فیلتر نتایج به سال:
This work describes the synthesis and use of the chelator lipid, nitrilotriacetic acid ditetradecylamine (NTA-DTDA). This lipid is readily dispersed in aqueous media, both alone and when mixed with carrier lipids like dimyristoylphosphatidylcholine (DMPC). Fluorescence microscopic examination of membranes deposited from NTA-DTDA-containing liposomes shows that NTA-DTDA mixes uniformly with the ...
Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested...
FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...
Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...
The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...
This year marks the 100th anniversary of Edison's invention of the electric lamp, one of many heralded accomplishments that brought him lasting fame. For much of life Edison enjoyed a popular reputation as a laboratory genius who personified the spirit of scientific discovery. Was he really a scientist, or only an inventor? His participation in the Draper Expedition to the solar eclipse of 1878...
The physician's art is the catalogue of an exhibition of over 100 images and other objects from the collections of four North Carolina medical schools. In a finely-judged Preface, structured around specific examples shown, Martin Kemp points out some of the historical issues involved in the study of medical imagery and artifacts, beginning with the problem of what "realism" means in the context...
How might a novel play tennis? In New York Times essay on Roger Federer, David Foster Wallace reflects how the climactic topspin lob that won Wimbledon for Federer in 2006 was product of narrative, reliant sequence bluffs, building up to final unreturnable shot whose genius lay its ‘unimaginable angle.’ Tennis becomes fiction here, both sense is unreal and it Rafael Nadal’s imagination ultimate...
JOHN HUXHAM, M.D., F.R.S., F.R.C.P. Edinburgh (c. 1692-1768), was an English physician whose practice was confined to Plymouth but whose writings were read throughout Europe. His literary career started with his Observationes de aere et morbis epidemicis (1739), in which meteorological records made at Plymouth from 1728 to 1737 were collated month by month with the diseases observed there. Furt...
from its emergence till present time marxist principles has undergone great changes since their promulgation by marx. many theorists and thinkers set at rectifying marxist tenets and introducing those of their own while others advocated its main concepts and attempted at improving them. among marxist philosophers who had an intensive study of marxs ideology, is louis althusser whose reflections...
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