Treatment of cultured bovine adrenal chromaffin cells with the catecholamine transport blocker reserpine was shown previously to increase enkephalin levels severalfold. To explore the biochemical mechanism of this effect, we examined the effect of reserpine treatment on the activities of three different peptide precursor processing enzymes: carboxypeptidase E (CPE) and the prohormone convertase...

Serotonin (5-HT) has been implicated as a potential modulator of the bystander effect in cell cultures. To assess the relevance of serotonin in vivo experiments were done with the zebrafish (Danio rerio). This species, when irradiated, transmits bystander signals to non-irradiated fish. The animals were injected with reserpine, an inhibitor of serotonin at a dose of 80mg/kg of body mass. The re...

The kinetics and thermodynamics of the adsorption process of reserpine adsorbed onto the strong acidic cationic exchange fiber (SACEF) were studied by batch adsorption experiments. The adsorption capacity strongly depended on pH values, and the optimum reserpine adsorption onto the SACEF occurred at pH = 5 of reserpine solution. With the increase of temperature and initial concentration, the ad...

noradrenaline uptake, had little effect (16% of inhibition). Transport was also dependent on aerobic metabolism: incubations with KCN ( 1 mM) or 2,4-dinitrophenol (1 mM) caused a 50% decrease. Egect of reserpine on the accumulation of I 'Hldopamine. Reserpine had no inhibitory effect on the accumulation of I 3H ldopamine after 1 day in uitro. At day 3, reserpine ( I O ~ M ) inhibited the 30-min...

On the basis of studies on cardiac tissue removed from animals treated with antiadrenergic drugs, a number of investigators have suggested that the positive inotropic response to digitalis requires norepinephrine in the cardiac muscle. In the present study the action of strophanthidin was studied in isolated papillary muscles obtained from normal cat hearts, from chronic, totally cardiac-denerv...

Dopaminergic denervation supersensitivity has been implicated in the pathogenesis of levodopa-induced dyskinesias, the most common and limiting side effect in the drug treatment of Parkinson's disease, yet the mechanisms that mediate altered drug sensitivity remain poorly understood. In animals models, one key component of denervation supersensitivity is the enhanced efficacy of selective D1 ag...

To justify the use of a drug in the routine treatment of hypertension it should be capable of reducing the diastolic and systolic blood pressures by 20 mm. Hg or more throughout the day and tolerance to its action should not develop on prolonged administration; the therapeutic dose should be considerably less than the toxic dose and since indefinite administration is required, the sideeffects s...

SUMMARY The effect of guanethidine and reserpine on the reflex sympathetic nervous system vasoconstriction in the calf and foot induced by a prolonged, generalized stimulus (body cooling for 1 hour) was studied in 12 normal subjects. Large daily clinical-doses of guanethidine (40 to 60 mg) or reserpine (1 mg) were given orally for 2 and 3 weeks, respectively. Foot and calf blood flows were meas...