Forensic DNA analysis has vastly evolved since the first forensic samples were evaluated by restriction fragment length polymorphism (RFLP). Methodologies advanced from gel electrophoresis techniques to capillary and now next generation sequencing (NGS). Capillary was still is standard method used in analysis. However, dependent upon information needed, there are several different that can be t...

PURPOSE To investigate the degree of heteroplasmy of the 11778 mtDNA mutation in Chinese patients with Leber's hereditary optic neuropathy (LHON). METHODS Seventeen Chinese Leber's pedigrees, including 24 patients, 17 unaffected maternal lineages, 4 internal controls, and 6 unrelated controls, were screened for the 11778 mtDNA mutation. This was carried out by analysis of the restriction frag...

background: aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, na­sal sinusitis and invasive infection. in this study, we developed a pcr-single strand confomational polymorphism method to identify the most common aspergillus species and we showed some advantages of this method comparing a pcr-restric­tion fragment length polymorphism with ...

Genetic interrelatedness among 13 strains of mycoplasmalike organisms (MLOs) from various sources was evaluated by dot hybridization and restriction fragment length polymorphism analyses using cloned DNA probes derived from Canadian peach X (CX) and western X (WX) MLOs. Dot hybridization analysis indicated that CX, WX, and clover yellow edge MLOs are closely related and form a distinct strain c...

The ability to recover sufficient amounts of deoxyribonucleic acid (DNA) from various biological evidentiary samples for individualization purposes is discussed. The chemistry and stability of DNA under various conditions is reviewed. The recovery of nondegraded DNA bears directly on the ability to successfully analyze such samples by restriction fragment length polymorphism analysis, as well a...

Techniques were developed for genetic characterization of Legionella pneumophila serogroup 1 by using restriction fragment length polymorphism analysis. Allozyme analysis provided an index of the discrimination achieved by restriction fragment length polymorphism. Isolates from human cases of legionellosis were examined by both methods, and their profiles were compared with reference strains of...

Previous cytogenetic studies suggest that various rDNA chromosomal loci are not equally active in different cell types. Consistent with this variability, rDNA polymorphism is well documented in human and mouse. However, attempts to identify molecularly rDNA variant types, which are regulated individually (i.e., independent of other rDNA variants) and tissue-specifically, have not been successfu...

purpose: to investigate the effect of ccl2c-2512t polymorphism on age-related macular degeneration (amd). methods: this study enrolled 48 patients originating from northeast of iran who suffered from dry type amd. the association of c-2518t in the promoter region of ccl2 gene was investigated using polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp). control grou...