In this study the restriction endonuclease analysis (REA) technique was used for the comparison of field isolates and vaccinal strain of Pasteurella multocida using Hha-I enzyme. On the basis of DNA finger prints, extent of homology among the field isolates and the vaccinal strain was observed. The study has provided the clear evidence that eight isolates of P. multocida used were serologically...

Three approaches were used to identify DNA polymorphisms in humans by examining the DNAs of a number of individuals within Huntington's Disease pedigrees as well as random individuals for restriction fragment length polymorphisms (RFLPs). The first approach used randomly chosen, cloned single-copy DNA segments from four types of human recombinant DNA libraries as hybridization probes to detect ...

13 14 High-throughput sequencing of reduced representation libraries obtained through digestion with 15 restriction enzymes – generally known as restriction-site associated DNA sequencing (RAD-seq) – is now 16 one most commonly used strategies to generate single nucleotide polymorphism data in eukaryotes. The 17 choice of restriction enzyme is critical for the design of any RAD-seq study as it ...

We developed a simple, rapid and reliable method to delete DNA fragments in plasmids using a polymerase chain reaction based amplification of the circular DNA sequence that excludes the fragment to be deleted. The primers are designed to contain a non-complementary 5' sequence consisting of a restriction enzyme target sequence. Following PCR amplification, the plasmid is digested with Dpn I to ...

b-lactoglobulin (Coded by the b-lg gene) is the major milk whey protein in ruminants. Studies have shown that this protein is polymorphic in many breeds of sheep as a result of a single base pair substitution in the b-lg gene that also gives rise to RsaI restriction fragment length polymorphism (RFLP). Blood samples were collected from 391 animals belonging to 5 Iranian and 6 Russian sheep bree...

Background: Toxoplasmosis, caused by Toxoplasma gondii, is a common parasitic disease, affecting almost one-third of the world’s population. It is transmitted by ingestion of food or water contaminated with oocysts excreted by cats and the consumption of raw or undercooked meat from ruminants. This study aimed at molecular characterization of T. gondii in native cattle from West of Tehran, Iran...

To generate DNA deletions, a tandem array of class IIS restriction enzyme recognition sites was cloned into a plasmid. The recognition sites were arranged so that each enzyme cleaves at a different site within an adjacent target sequence. Digestion with both enzymes followed by end repair and ligation resulted in the deletion of DNA between the two sites of cleavage. Because both recognition si...

Most restriction endonucleases use Mg2+ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification reactions at its recognition site. In the presence of alcohols such as ethanol or glycerol, it attaches the alcohol covalently to the 5' terminus...

Type I restriction-modification (RM) systems are comprised of two multi-subunit enzymes, the methyltransferase (∼160 kDa), responsible for methylation of DNA, and the restriction endonuclease (∼400 kDa), responsible for DNA cleavage. Both enzymes share a number of subunits. An engineered RM system, EcoR124I(NT), based on the N-terminal domain of the specificity subunit of EcoR124I was construct...