نتایج جستجو برای: reverse transcription pcr rt
تعداد نتایج: 497860 فیلتر نتایج به سال:
A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 gen...
Abstract Background: Factor VII, is a coagulant protease it begins the proteolytic cascade reactions and produces thrombin. The use of recombinant human factor VII, (rhFVII) is effective for the treatment of patients with hemophilia A or B. It is a target for gene therapy. This study was done to clone factor VII from HepG2 cell line. Methods: RNA was extracted from the hepatoma, (HepG2), ...
soybean mosaic virus (smv) which belongs to the virus family potyviridae, causes a disease in soybean that is present in soybean-growing areas of the world, and is widely distributed in northern iran. detection of smv is very important for disease management. in the present study several serological and molecular (nucleic acid- based) methods of rapid virus detection were compared. serological ...
in this study, the possible infection of thomson navel, moro blood sweet orange, page mandarin and webber pomelo with citrus viroids was investigated. biological indexing was performed by grafting on etrog citron (c. medica) and maintained in temperature-controlled greenhouse. after inoculation, rna was extracted by sds– potassium acetate method. detection of citrus viroids was done reverse tra...
BACKGROUND In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression. METHODS We used SYBR green I-based quantitative real-time PCR (QPCR) to measure the properties of reverse transcription reaction for the beta-tubulin, glyceraldehyde-3-phosphate deh...
Various approaches can now be taken for amplification of RNA transcripts using the polymerase chain reaction (PCR). Here, we compare three such methods: (i) uncoupled reverse transcription (RT)-PCR (using separate reactions for cDNA synthesis and PCR), (ii) continuous RT-PCR (in which RT and DNA amplification occur in an uninterrupted reaction) using either a single enzyme for both RT and DNA a...
Merkel cell polyomavirus (MCPyV) is the etiological agent of majority carcinoma (MCC): a rare skin tumor. To improve our understanding role MCPyV in MCCs, detection and analysis DNA transcripts were performed on primary tumors regional lymph nodes from two MCC patients: one metastatic non-metastatic. MCPyV-DNA was searched by quantitative polymerase chain reaction (qPCR), followed amplification...
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...
Human metapneumovirus (hMPV) is an etiologic agent of respiratory tract infections. In this study, we compared the sensitivity and specificity of real-time reverse transcription (RT)-polymerase chain reaction (PCR), conventional RT-PCR, and nested PCR in detecting hMPV genes. A total of 146 clinical specimens from 143 patients who showed acute respiratory tract infection symptoms were tested by...
OBJECTIVE To identify transcriptionally active open reading frames (ORFs), predicted by bioinformatics, within RD1 genomic segment of Mycobacterium tuberculosis using reverse transcription-polymerase chain reaction (RT-PCR). MATERIALS AND METHODS M. tuberculosis H37Rv was grown in Middlebrook 7H9 medium for 8 weeks and total RNA was isolated using standard procedures. The cDNA was synthesized...
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