A Myceliophthora thermophila laccase and a Rhizoctonia solani laccase were mutated on a pentapeptide segment believed to be near the type-1 Cu site. The mutation L513F in Myceliophthora laccase and the mutation L470F in Rhizoctonia laccase took place at a position corresponding to the type-1 Cu axial methionine (M517) ligand in Zucchini ascorbate oxidase. The triple mutations V509L,S510E,G511A ...

Root rot is an important and widespread disease that often causes significant reduction in the yield and quality of harvested peas throughout the production areas in New York. Pea root rot can be caused by one of several soilborne pathogens or various combinations of root pathogens and also non-pathogens. In recent years, Fusarium solani f. sp. pisi (causal pathogen of Fusarium root rot) has be...

An experiment was conducted to find out variation in isolated Rhizoctonia solani based on radial mycelial growth and sclerotial production. Five isolates of Rhizoctonia solani representing five clusters group were selected and were grown at different levels of temperature and pH on potato dextrose agar (PDA). It was observed that optimum temperature and pH for growth and scierotial production v...

In disease-suppressive soils, plants are protected from infections by specific root pathogens due to the antagonistic activities of soil and rhizosphere microorganisms. For most disease-suppressive soils, however, the microorganisms and mechanisms involved in pathogen control are largely unknown. Our recent studies identified Actinobacteria as the most dynamic phylum in a soil suppressive to th...

Antifungal activity of ethanol-water extracts of four medicinal plants, cinnamon (Cinnamomum verum Presl.), anise (Pimpinella anisum L.), black seed (Nigella sativa L.) and clove (Syzygium aromaticum L. Merr. & Perry.) was investigated against pea (Pisum sativum L.) root-rot fungus Rhizoctonia solani. In vitro antifungal activity test shown a high growth inhibition at concentration (4%) of each...

biological control of sugar beet damping-off of rhizoctonia solani ag-4 by two native streptomyces isolates (s2 and c) was evaluated in green house and field conditions. in dual culture, two streptomyces isolates inhibited mycelial growth of r. solani ag-4. besides, volatile components of s2 and c inhibited r. solani mycelial growth for 72 and 74 percentages, respectively. soil treatment either...