The successful treatment by riboflavin of a number of cases of superficial keratitis was described in two previous papers (Aykroyd and Verma, 1942; Verma, 1942). In the course of this work it was observed that a number of patients with superficial keratitis, associated with angular stomatitis and other signs of riboflavin deficiency, were also suffering from angular conjunctivitis, and that the...

An improved method is presented for the purification of 8 alpha-(N1-histidyl)riboflavin, 8 alpha-(N3-histidyl)riboflavin and their 2',5'-anhydro forms, which permits the isolation of sizeable quantities of each of these compounds from a synthetic mixture in pure form. Flavin peptides were isolated from the D-gluconate dehydrogenases of Pseudomonas aeruginosa and Pseudomonas fluorescens and from...

The production of liver tumors in the rat by the oral administration of N,N-dimethyl-p-aminoazobenzene (DMB) is markedly influenced by diet. Our early work (1,2) showed that the maintenance of normal hepatic riboflavin levels was of importance in protecting rats against tumor formation. The work of Griffin and Baumann (3) and Miller, Miller, Kline, and Rusch (4) has emphasized the relationship ...

BACKGROUND Meta-analyses predict that a 25% lowering of plasma homocysteine would reduce the risk of coronary heart disease by 11% to 16% and stroke by 19% to 24%. Individuals homozygous for the methylenetetrahydrofolate reductase (MTHFR) 677C-->T polymorphism have reduced MTHFR enzyme activity resulting from the inappropriate loss of the riboflavin cofactor, but it is unknown whether their typ...

PURPOSE To correct for attenuation in two-photon fluorescence (TPF) measurements of riboflavin absorption in porcine corneas. METHODS Two-photon fluorescence imaging of riboflavin was performed using excitation at a wavelength of 890 nm, with fluorescence signal detected between 525 and 650 nm. TPF signal attenuation was demonstrated by imaging from either side of a uniformly soaked corneoscl...

The livers of rats fed 10 diets previously found to inhibit or accelerate the induction of tumors by 4-dimethylaminoazobenzene were analyzed for riboflavin, biotin, and vitamin B(6) after 6 and 19 weeks on the diets. Those fed diets accelerating tumor induction had average hepatic riboflavin levels of 9 to 11 microg. per gm. The livers of the control and protected rats averaged 14 and 15 to 19 ...

Though the synthesis of riboflavin in large amounts by microorganisms has been encountered, very little is known about the mechani involved in the biosynthesis of this vitamin. A critical examination of the various environmental conditions for different organisms producing this vitamin showed that the optimum conditions for the production of riboflavin varied widely from organism to organism (S...

Complex B vitamins as Biotin and Riboflavin are required by living organisms, not only for growth but also for metabolite production, and the feed market classifies them as growth promoters. Since Brazil will soon be one of the world's biggest animal protein producers, feed production is a large consumer of vitamins and micronutrients. The industry requires 10 mg riboflavin/0.2 mg biotin per ki...

NLY two riboflavin mutants have been described so far in Neurospora crassa, 51602t(rib-I) (MITCHELL and HOULAHAN 1946) in linkage group VI, and Y30539 (rib-2) (GARNJOBST and TATUM 1956) in group IV. MCNUTT and FORREST (1958) list two other riboflavin mutants which were used in testing their pteridine derivative from Eremothecium as a growth substance. One of these is 76R5 (C168, METZENBERG, per...

Multiple acyl-CoA dehydrogenation deficiency (MADD) is a disorder of fatty acid, amino acid and choline metabolism that can result from defects in two flavoproteins, electron transfer flavoprotein (ETF) or ETF: ubiquinone oxidoreductase (ETF:QO). Some patients respond to pharmacological doses of riboflavin. It is unknown whether these patients have defects in the flavoproteins themselves or def...