Clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease 9 (CRISPR-Cas9) system was instigated first into eukaryotes in the last ten years are becoming productive and worldwide application for genome modification. Genome engineering through insertion of foreign DNA insert have numerous disadvantages which could be overcome by use DNA-free editing. Various ways editin...

Using immunochemical techniques, we have examined the macromolecular state of association of the major heterogeneous nuclear ribonucleoprotein (hnRNP) core proteins in mitotic HeLa cells. We find that these proteins are not free but are associated with high-molecular-weight RNA in the form of particles that sediment as a broad band between 80 and 200 S. We have termed these complexes MhnRNP for...

A new paradigm is emerging in plant biology in which proteins and ribonucleoprotein (RNP) complexes play non-cellautonomous roles in contributing to the control over developmental and physiological processes. Plasmodesmata (PD), the intercellular organelle(s) of the plant kingdom, create the pathway for the cell-to-cell trafficking of these information macromolecules. This functional property o...

The structure of the ribonucleoprotein (RNP) complex of three coronaviruses was investigated. A single-stranded helix of diam. 14 to 16 nm and up to 320 nm in length was released from disrupted particles of human coronavirus strain 229E and mouse hepatitis virus strain 3 after incubation in mild conditions. The helical complexes appeared to be composed of globular subunits with long axes of 5 t...

The present work was undertaken to study the distribution of yeast nucleoprotein in cell fractions after centrifugal separation, and to define conditions of extraction and purification in order to obtain the nucleoprotein in a relatively undegraded state. Bakers’ yeast (Xaccharomyces cerevisiae) is the classical source of ribonucleic acid (RNA), but relatively little work has been reported on t...