The present study is carried out to analyze the trace elements sodium, calcium, magnesium, phosphorus, iron, copper, zinc, manganese by atomic absorption spectrometer (AAS) crude protein by Kjeldahl method and essential and non-essential amino acids by reverse phase high performance liquid chromatography (RP-HPLC) from feathers of cock (Gallus domesticus) before the administration of four ACES ...

Objective: To develop a rapid, accurate, linear, sensitive and stability indicating RP-HPLC method for the determination of nilotinib in bulk and pharmaceutical dosage forms in the presence of its four related substances. Methods: The RP-HPLC method was developed for the chromatographic separation of nilotinib and its impurities by using waters Xterra RP-18 (150*4.6 mm, 3.5 μm) column with a mo...

H and C NMR spectra were recorded with Bruker DPX-400, AVANCE-400 and DRX-500. The numbering of the protons relate to the numbering chosen in Fig. 1. High resolution mass spectra were obtained with a Micromass LCT via loop-mode injection from a Waters (Alliance 2695) HPLC system. Alternatively a Micromass Q-TOF in combination with a Waters Aquity Ultraperformance LC system was employed. Ionizat...

Log Kow values for 14 heterocyclic nitrogen compounds (by traditional shake-flask method) were reported. Reverse phase–high-performance liquid chromatography (RP-HPLC) method and three estimation models—linear solvation energy relationship, molecular connectivity indices (MCIs), and quantum chemical descriptors—were used to study the partition mechanism and to establish the correlations of the ...

The review of the RP HPLC analysis of basic psychotropic drugs is presented. It contains sample preparation methods with centrifugation, protein precipitation, liquid-liquid extraction (LLE), dispersive liquid-liquid microextraction (DLLME), solid-phase extraction (SPE), solid-phase microextraction (SPME), microwave-assisted extraction (MAE) and RP-HPLC analysis. Chromatographic behavior of bas...

Antibacterial peptides were isolated from immune haemolymph of Rhinoceros beetle (Oryctes rhinoceros L.) larvae and evaluated for antibacterial activity against bacterial pathogens, viz., Klebsiella pneumoniae, Micrococcus luteus, Bacillus subtilis, Paenebacillus macerans and Pseudomonas sp. The peptides were found to be stable upto 37°C and antibacterial activity observed at pH 7.0. The peptid...

A simple, specific, accurate, precise and reproducible method has been developed and validated for the estimation of Dithranol in lipid nanoparticle by RP-HPLC method. RP-HPLC estimation of dithranol in prepared formulation was performed using Phenomenex ODS 5μ, C18 column and Acetonitrile: Methanol: Buffer (20:20:60) as mobile phase which had shown sharp peak when detected at 258 nm. The linea...

In the present study, an improved method termed negative-pressure cavitation extraction (NPCE) followed by reverse phase high-performance liquid chromatography (RP-HPLC) was developed for the extraction and quantification of vindoline (VDL), catharanthine (CTR), vincristine (VCR) and vinblastine (VLB) from Catharanthus roseus leaves. The optimized method employed 60-mesh particles, 80% ethanol,...

A simple stability indicating reversed-phase HPLC method was developed and subsequently validated for estimation of Cefpirome sulphate (CPS) present in pharmaceutical dosage forms. The proposed RP-HPLC method utilizes a LiChroCART-Lichrosphere100, C18 RP column (250 mm × 4mm × 5 μm) in an isocratic separation mode with mobile phase consisting of methanol and water in the proportion of 50:50 % (...

A fast, specific, and sensitive method for determination of soy additives in processed meat products has been developed. The method is based on RP-HPLC analysis of the isoflavones daidzein and genistein using bisphenol A as an internal standard. This paper describes the development and optimisation of efficient extraction procedures (n-hexane/80% EtOH), concentration of isoflavones by solid-pha...