1. Four rumen-cannulated sheep were given a forage mixture (F) of chopped hay-ground, pelleted, dried grass (92:8, w/w) and two concentrate mixtures (C and S) of ground barley-ground hay-flaked maize (46:24:30 and 56:24:20, by wt respectively) in twenty-four hourly meals each day. Each of the diets was offered in successive periods of 16 d to give a feeding sequence F-S-C-S for one pair of shee...

Four in vitro experiments (Exp.) were conducted to evaluate lavender essential oil (LEO) effects at 0 (control), 250 (low dose), 500 (medium dose), 750 and 1000 µL per L(high doses) of incubation medium on rumen gas production kinetics (Exp.1), ruminal digestibility and fermentation (Exp.2), methane production (Exp.3) and rumen acidosis (Exp.4). The asymptote of gas production (A) incr...

the aim of this study was to assess the effect of several doses of spearmint essential oil (seo; 0, 250, 500, 750 or 1,000 µg ml-1 buffered rumen fluid) on the fermentation kinetic and digestibility using in vitro gas production technique. a total mixed ration (30% roughage: 70% concentrate) was incubated with buffered rumen fluid. in vitro gas production, asymptotic gas production (a), rate of...

this experiment was conducted to investigate the effects of garlic powder on fermentation characteristic and rumen microbes of arabian sheep.at first step the best level of garlic was determined. then animals were fed for 35 days with control diet and supplemented with garlic (2% per kg dm). apparent digestibility, rumen fermentation parameters, number of protozoa and blood metabolites (glucose...

The negative effect of pH on rumen microbial fermentation has been associated with the total amount of time that pH is below a certain threshold. However, not only the time, but also the magnitude of the pH reduction, is important. Eight 1,325-mL dual-flow continuous culture fermenters were used in 2 replicated periods to determine the effect of the magnitude of pH reduction (5.6 vs. 5.1) durin...

The effect of silage additives on ensiling characteristics and nutritive value of Napier grass (Pennisetum purpureum) silages was studied. Napier grass silages were made with no additive, fermented juice of epiphytic lactic acid bacteria (FJLB), molasses or cassava meal. The ensiling characteristics were determined by ensiling Napier grass silages in airtight plastic pouches for 2, 4, 7, 14, 21...

The effect of replacing concentrate with 2 different feed blocks (FB) on ruminal fermentation and microbial growth was evaluated in goats and in single-flow continuous-culture fermenters. Diets consisted of alfalfa hay plus concentrate and alfalfa hay plus concentrate with 1 of the 2 studied FB. Three trials were carried out with 6 rumen-fistulated Granadina goats and 3 incubation runs in 6 sin...

Management of metabolic hydrogen ([H]) in the rumen has been identified as an important consideration when reducing ruminant CH4 emissions. However, little is known about hydrogen flux and microbial rumen population responses to CH4 inhibition when animals are fed with slowly degradable diets. The effects of the anti-methanogenic compound, chloroform, on rumen fermentation, microbial ecology, a...

in this experiment, different doses of zataria multiflora (zm) and eucalyptus globolus (eg) essential oils (eos) (0, 100, 250, 500, 750 and 1000 µg/ml) and a mixture of them were added to alfalfa hay incubated with buffered rumen liquor of merghoz goat to assess in vitro gas production, rumen fermentation and protozoa population. in all treatments the asymptotic gas volume (b) and the rate cons...

Nutritive values of green and black tea by-products and anti-nutritive activity of their tannins were evaluated in an in vitro rumen fermentation using various molecular weights of polyethylene glycols (PEG), polyvinyl pyrrolidone (PVP) and polyvinyl polypyrrolidone as tannin-binding agents. Significant improvement in gas production by addition of PEG4000, 6000 and 20000 and PVP was observed on...