The majority of workers are agreed that the digestion of carbohydrate in the rumen is brought about by the micro-organisms living there, and furthermore, that the microflora plays a much larger part in these processes than the microfauna, for if the Protozoa are removed by drenching with dilute copper sulphate, the digestive processes continued unhampered (see Van der Wath, 1942). The precise r...

Microorganisms in the digestive tract of ruminants differ in their functionality and ability to use feed constituents. While cecal microbiota play an important role in post-rumen fermentation of residual substrates undigested in the rumen, limited knowledge exists regarding its structure and function. In this trial we investigated the effect of dietary supplementation with linseed oil and nitra...

Two metabolism trials (experiments 1 and 2) were conducted to examine the effect of the organic S compound, sodium 3-mercapto-1-propane sulfonic acid (MPS) on feed intake, fiber digestibility, rumen fermentation and abundance of cellulolytic rumen microorganisms in cattle fed low S (<0.11%) roughages. Urea was provided in all treatments to compensate for the N deficiency (<0.6%) in the roughage...

Cattle manure (CM) is mostly used as an inoculum in order to start-up of agricultural biogas plants or a co-substrate the anaerobic digestion lignocellulosic content. Rumen fluid microbiota also considered be effective lignocellulose digestion. It known that microorganisms ruminal waste facilitate hydrolysis structures. However, there are few studies on effect rumen bioreactor performances when...

abstract             the aim of this study was to evaluate the effect of ajowan essential oils (aeo) on rumen fermentation using in vitro gas production. therefore, different doses of aeo (i.e., 0, 150, 300, 450 or 600 ppm) were added to the syringes of the gas production. a typical diet of growing lambs was used as fermentation substrate.  at the first step, the gas production syringes were in...

The importance of microorganisms to the mixed activities could be obtained. For this ruminant has interested an increasing number of reason, and because the pure culture approach nutritionists, particularly those in departments is time consuming, methods for studying the of animal science. In view of this interest of natural mixture have been developed. The inagricultural workers, it is surpris...

this study was carried out to determine the potential of rumen microorganisms in samples taken from slaughterhouse to produce fibrolytic enzymes and to compare different methods for extraction, concentration and conservation of them.  according to the results, the average specific activity of cellulase and xylanase were 7.5 and 16.5 u/mg protein respectively. the application of homogenizing and...

Recent advances in silage microbiology are reviewed. Most new techniques in silage microbiology use the polymerase chain reaction (PCR) to make copies of a portion of the DNA in microorganisms. These techniques allow us to identify and quantify species as well as do community analysis. The PCR-based techniques are uncovering new species, both bacteria and fungi, during storage and feeding. Sila...

Some fatty acids (FAs) can inhibit or stimulate the growth of bacteria. In general, polyunsaturated FAs such as linoleic and linolenic acids have more inhibitory effects on bacteria than saturated and monounsaturated FAs (Nieman, 1954; Raychowdhury et al., 1985). Gram-positive bacteria, especially, are more sensitive to polyunsaturated FAs than Gram-negative bacteria (Greenway and Dyke, 1979). ...

Ruminants rely on a complex rumen microbial community to convert dietary plant material to energy-yielding products. Here we developed a method to simultaneously analyze the community's bacterial and archaeal 16S rRNA genes, ciliate 18S rRNA genes and anaerobic fungal internal transcribed spacer 1 genes using 12 DNA samples derived from 11 different rumen samples from three host species (Ovis a...