Subclinical infections of the mammary gland and reproductive tract of dairy cattle cause significant production losses through decreased milk yield and lowered fertility. in the absence of overt signs of infection, there are no simple, reliable, diagnostic tools to accurately identify cows with these conditions, some of which may progress to a clinical stage at a later date. A search was undert...

A rapid and efficient DNA extraction method was developed for detecting mastitis pathogens in milk. The first critical step involved cell wall disruption by bead-beating, as physical disruption using beads was more effective for DNA extraction from Gram-positive bacteria, such as Staphylococcus aureus, than enzymatic disruption using proteinase K. The second critical step involves the use of ac...

Cuvinte cheie: alergie, proteine lapte vacă, toleranţa orală Rezumat: Alergia la proteinele din laptele de vacă este definită ca reacţia imunologică, mediată sau nemediată IgE, la proteinele din laptele de vacă. Afectează populaţia pediatrică în primii 2 ani de viaţă, după această vârstă instalându-se toleranţa la proteinele din laptele de vacă. Sistemul imun intestinal este răspunzator de iniţ...

s and proceedings published in international journals : Hajós Gy., Gelencsér É., Szerdahelyi E., Bardocz S., Pusztai A.: Enzymatic modification as a tool for improving the quality of food proteins in: "COST 98 Effects of antinutrients of the nutritial value of legume diets" (Eds: S. Bardocz, A. Pusztai) Official Publications of the European Communities, Brussels, 1, 69-74 (1996) Hajós Gy., Gele...

Analyses of the rat mammary gland show that the i n c r e a s e in the milk-protein mRNAs during the development of lactation and the rapid disappearance of these sequences during involution are not accompanied by similar changes in the poly(A) content. During the development of lactation the casein mRNA is initially in great excess to the whey-protein mRNA and this differential expression of t...

Human milk contains many proteins that have been shown to be bioactive, but it is still not known whether these activities are exerted in breast-fed infants. These bioactivities include enzyme activities, enhancement of nutrient absorption, growth stimulation, modulation of the immune system and defence against pathogens. The antimicrobial activities are very diverse, ranging from stimulation o...

Caseins were separated from whey proteins by acid precipitation of skimmed rabbit milk. Whole casein was resolved by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis into three major bands with apparent relative molecular masses (Mr of 31 000, 29 000 and 25 000. On agarose/urea-gel electrophoresis whole casein gave three bands with electrophoretic mobilities a, , and y. The three comp...

The aim of the present study was to investigate the protective effects of camel milk on hepatic pathogenicity induced by experimental infection with Escherichia (E. coli) and Staphylococcus aureus (S. aureus) in Wistar rats. The rats were divided into six groups: The control and camel milk groups received water and camel milk, respectively; two groups received camel milk for 2 weeks prior to in...

A rapid and efficient DNA extraction method was developed for detecting mastitis pathogens in milk. The first critical step involved cell wall disruption by bead-beating, as physical disruption using beads was more effective for DNA extraction from Gram-positive bacteria, such as Staphylococcus aureus, than enzymatic disruption using proteinase K. The second critical step involves the use of ac...

Benefits of breastfeeding are widely recognized, during the last decades human milk has been identified as the normative standard for infant feeding and nutrition. Recent evidence focused on specific bioactive and immunomodulatory factors, such as oligosaccharides, lactose, glycosaminoglycans of human milk and the variability of their concentrations during lactation in both term and preterm mil...