Ingunn Ulrich and Wolfgang Ulrich Institut für Genetik. Universität Hohenheim , Garbenstraße 30, D-7000 Stuttgart-Hohenheim , Bundesrepublik Deutschland Z. Naturforsch. 41c, 1052 — 1056 (1986); received July 23, 1986 Flow Cytometry, Plant Protoplasts, Protoplasts Preparation, D N A , D A PI Protoplasts prepared from leaves of various higher plants were stained with the specific DNA fluorochrome...

We consider a cell sorting process based on negative dielectrophoresis. The goal is to optimize the shape of an electrode network to speed up the positioning. We first show that the best electric field to impose has to be radial in order to minimize the average time for a group of particles. We can get an explicit formula in the specific case of a uniform distribution of initial positions, thro...

fiuorescent spot in the A. nidulans transformant, approximately 66% of conidia had two, 24% had one, and 10% had three or more in the A. oi:yzae transformant. The conidia expressing H2B::EGFP werc put through FACS (fluorescence-activated cell sorting> analysis and two sharp peaks, corresponding to one and two nuclei in each co"idium, were noted in the A. ot:yzae transformant. In addition, the A...

The isolation of haploid cell lines has recently allowed the power of forward genetic screens to be applied to mammalian cells. The interest in applying this powerful genetic approach to a mammalian system is only tempered by the limited utility of these screens, if confined to lethal phenotypes. Here we expand the scope of these approaches beyond live/dead screens and show that selection for a...

Abstract.We demonstrate how concepts of statistical mechanics of interacting particles can have important implications in the choice of interaction potentials to model qualitative properties of cell aggregates in theoretical biology. We illustrate this by showing cell sorting phenomena for cell groups with different adhesiveness parameters: ranging from well-mixed cells aggregates to full segre...

The ability to routinely generate efficient protein catalysts of bond-forming reactions chosen by researchers, rather than nature, is a long-standing goal of the molecular life sciences. Here, we describe a directed evolution strategy for enzymes that catalyze, in principle, any bond-forming reaction. The system integrates yeast display, enzyme-mediated bioconjugation, and fluorescence-activate...

We aimed to improve the efficiency of isolating endometrial epithelial and stromal cells (EMECs and EMSCs) from the human endometrium. We revealed by immunohistochemical staining that the large tissue fragments remaining after collagenase treatment, which are usually discarded after the first filtration in the conventional protocol, consisted of glandular epithelial and stromal cells. Therefore...

DOI: 10.1002/elps.202100057 The cover picture shows sorting of microdroplets using the sequentially addressable dielectrophoretic array (SADA). SADA is an electrodes (as shown in gold picture) that generates localized electric field synchronized to position and speed flowing target droplets indicated by blue curves picture). resulting force displaces only from default flow, enabling separation ...