Low molecular weight organic acids are most predominant in apple juice among these components fumaric acid was not exceed more than 5 ppm. It was thought that the HPLC is the best method of the determination of organic acids in the apple juice. In this study, chromatographic separation of organic acids of apple juice was obtained by preparing a sample and by applying them to acid phase for ...

Chemometric methods like Principal Component Analysis (PCA) and Evolving Factor Analysis (EFA) have been applied to improve peaks separation, especially in HPLC UV-DAD analysis. In this work, the Independent Component Analysis (ICA) was adopted for the separation of overlapping aromatic peaks and the simultaneous determination of the underlying spectra. The application of the method on middle p...