OBJECTIVES The aim of this study was to determine the roles of mutations in the gyrA and parC genes and the overexpression of efflux pump(s) as mechanisms of resistance to quinolones. Forty-five Yersinia enterocolitica O:3 clinical isolates (41 nalidixic acid-resistant, three nalidixic acid-susceptible and one nalidixic acid-resistant strain obtained in vitro) were analysed. RESULTS All the n...

A number of antigens in unfertilized eggs and embryos of the sea urchin Paracentrotus lividus were characterized with respect to both immunological and physicochemical properties. Experiments involved single diffusion in agar (Oudin technique) combined with mutual dilution, serial dilution, and heating of antigenic extracts, as well as immunoelectrophoresis with normal and heated extracts and a...

introduction: gene expression and purification of luciferases from the firefly, lampyris turkestanicus, and optimization of cellular atp measurements were performed. methods: cdna encoding luciferases from lampyris turkestanicus was transferred from pqe30 vector into pet28a expression vector and pltu28 was built. newly constructed vector was expressed in e. coli xl1 blue and the recombinant luc...

abrin known as ribosome inactivating protein’s (rip) it is a high cytotoxic plant protein. high lethality and low cost and easy access to plant and seeds lead to this toxin used in crimes and terrorist acts. because obtaining purified toxins requires advanced laboratory equipment and complex procedures, so, it appears that the perpetrators of such crimes use crude extracts. as a result, remaini...

Tomato is the second most important vegetable crop. It widely grown on all over world. Diseases are major constraint in economic crop production as they inflict heavy loss tomato. Among various fungal diseases, early blight was reported severe it causes damage to The main aim of this study explore potentiality native and efficient isolate P. fluorescens biocontrol agent. A total 30 Pseudomonas ...

To investigate the seroprevalence of leptospiral infection in sheep in Ahvaz, southwestern Iran, bloodsamples were taken from 181 female sheep. Sera were stored at -20°C until use. They were initially screenedat serum dilution of 1:100 against six live antigens of Leptospira interrogans serovars pomona, canicola,hardjo, ballum, icterohaemorrhagiae, grippotyphosa using the microscopic agglutinat...

Abstract:   Objectives: Flexion contractures of the proximal interphalangeal joint are of the most common complication result from surgical procedures and traumatic events.  Orthotic interventions for treatment of contractures may include serial splinting, serial casting, dynamic and/ or static progressive orthoses, or a combination of these orthoses.  This study aimed to determine the effect ...

has been used for therapeutic purposes since ancient times. Different studies report its antimicrobial activity against an important human pathogen such as S. aureus. Therefore, in our study, we aimed to examine the efficacy of donkey milk standard MRSA and MSSA strains in-vitro environment. In obtained from 53 different donkeys aureus ATCC 43300 (MRSA) 29213 (MSSA) was investigated by broth mi...

resistance of streptococcus pneumoniae against penicillin is considered to be of great importance. while low-resistant strains could be treated by penicillin, treatment of highly resistant strains is very difficult and needs broad-spectrum antibiotics. this study was performed in imam khomeini medical center, tehran, iran, from 1999 to 2001 to evaluate pneumococcal resistance against penicillin...