It has been shown that enzymes in serum will change maltose, dextrin, and starch so that they will react as dextrose in media. These enzymes are destroyed by heating to 60 degrees C. for 15 minutes, but they are present in sera that have been refrigerated for as long as 18 months. The practice of using carbohydrate media containing unheated serum should be discouraged, and if it is used the pos...

The diagnostic value of cerebrospinal fluid (CSF) enzyme activities in neurological disorders has been evaluated most extensively with the enzymes aspartate aminotransferase (ASAT), lactate dehydrogenase (LDH), creatine kinase (CK) and lysozyme. Methods use for performing the assays have been similar to those employed for serum analysis. Reference intervals for these enzymes in the CSF are give...

The demonstration of tissue specific isoenzymes of lactic dehydrogenase and their variations in certain diseases (1, 2, 3) has invigorated the interest for further fractionation of other serum enzymes. Recently FLODIN and KILLANDER (4) have reported on the fractionation of human serum proteins by filtration through porous dextran gel "Sephadex G-200" (4). This method exposes the proteins to ver...

It has been shown that enzymes in serum will change maltose, dextrin, and starch so that they will react as dextrose in media. These enzymes are destroyed by heating to 60 degrees C. for 15 minutes, but they are present in sera that have been refrigerated for as long as 18 months. The practice of using carbohydrate media containing unheated serum should be discouraged, and if it is used the pos...

Gongronema latifolium (GL) is reported to have hepato-protective and nephron-protective ability. Diabetes associated with liver kidney damage. This work was designed evaluate the effect of ethanolic leaf extract on functions in Streptozotocin-induced diabetic rats. 40 Wistar rats both sexes (150g-200g) were divided into five (n=8). Group 1- normal control (received saline placebo orally). 2 rec...

Background Cell-free DNA circulates in cancer patients and induces in vivo cell transformation and cancer progression in susceptible cells. Based on this, we hypothesized that depletion of circulating DNA with DNAse I and a protease mix could have antitumor effects. Study design The study aimed to demonstrate that DNAse I and a protease mix can degrade in vitro DNA and proteins from the serum o...