The use of a single-radial-haemolysis technique for the detection of antibody to rubella virus is described. The single-radial-haemolysis test was compared with the standard HI methods for the detection of antibody to rubella virus. A close correlation between the two methods was observed in a survey of over two thousand serum samples and the study indicated that single-radial-haemolysis was hi...

Rat and rabbit IgG immunoglobulins conjugated to horseradiah peroxidase as a histochemical marker bind at 0 degrees C to the luminal surface of absorptive cells in isolated segments of jejunum from 10-12-day old rats. Binding is observed at pH 6.0, near the normal luminal pH of the duodenum and jejunum at this age, but not at pH 7.4. Furthermore, no binding occurs when cells are exposed at pH 6...

A patient with the hyperviscosity syndrome exhibited very high concentrations of intermediate to small circulating immune complexes (CIC), involving 40-50% of the IgG present, with IgG rheumatoid factor activity. We demonstrate that precipitation of CIC by polyethylene glycol in the reaction mixture caused interference with nephelometric methods for measuring IgM and IgA, and that failure of im...

-CLL is frequently accompanied by decreased levels of serum immunoglobulins which increases patients susceptibility to infections (1,2). Low levels of IgA, IgM and IgG occur in 30%, 30% and 10% of B-CLL patients respectively at the time of diagnosis. The appearance of decreased levels of immunoglobulins is a continuos process developing spontaneously during the untreated course of the disease. ...

Seven patients with gastrointestinal disorders and deficiencies of serum and secretory immunoglobulins were evaluated clinically, microbiologically, and immunologically. Five patients had generalized deficiencies of immunoglobulins; two were selectively deficient. Diarrhoea and malabsorption in six of the seven patients were at least partially explained. Four of the five patients with generaliz...

Plasma cell disorders are important in the differential diagnosis of acute kidney injury and of renal impairment with proteinuria. One or more of several mechanisms can be involved, such as cast nephropathy, deposition of monoclonal immunoglobulins, formation of amyloid fibrils, hypercalcaemia, hyperuricaemia and a variety of tubular syndromes. Various tests, such as serum electrophoresis and i...

Methods Prospective and observational study.We included patients older than 18 years old, admitted to ICU and undergoing mechanical ventilation, who have been presented an acute failure of unless two organs. At admission, and on day 3 and 7, we determined: -Immunophenotypic lymphocyte subpopulations (ILS) (T, B and NK), concentration of immunoglobulins in serum, and expression of HLA-DR on mono...

The study was conducted on 85 neonatals of Pak-Karakul sheep at the Sheep and Goat Development Centre, Rakh Khairewala, District Layyah, Pakistan, with the aim of assessing the relationship of immunoglobulins to neonatal lamb mortality. Overall mortality in neonates was 8.24%. Surviving lambs (3.77±0.07 kg) were significantly (P<0.001) heavier than those that died during the neonatal period (2....

The effect of immunoglobulins and complement (C) on phagocytosis and intracellular killing of Escherichia coli was studied in vitro. The incubation system consisted of monolayers of human polymorphonuclear leukocytes and C-resistant, [3H]thymidine-labeled E. coli C source was human serum deprived of immunoglobulins and properdin by immunoabsorption. In the absence of C, only immunoglobulin G-co...