Abstract This study was carried out in the Plant Tissue Culture Laboratory of College Agriculture - Karbala Unaiversity for period from 2022 to 2023. The included use explants and different combinations sterilization, initiation multiplication vegetative shoots Stevia plantiv vitro , three phases: first sterilization (shoot tips lateral buds) using sodium hypochlorite at concentrations (0, 0.5,...

Nowadays, many researches were conducted in minimizing tissue culture technology due to the overhead of cost needed. The purpose of this study was to investigate the effects of using five kinds of organic additives at four level concentrations responsive to the number of shoots produced for eight weeks in culture. Stem segment explants of Celosia sp. were cultured on MS medium that have been su...

punicagranatum cv. malassaveh is one of the most desirable commercial cultivars in iran. in thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. for thispurpose, both liquid and agarmurashige and skoog (1962) (ms) medium supplemented with baat 2, 4.5, 9, 13 μm alone and in...

background: management of canal isthmus is considered as an important factor for successful endodontic treatment. accordingly, this study was designed to determine the prevalence, location, and types of isthmus in mesial root canals of extracted mandibular molars in a sample of iranian population. materials and methods: in this cross-sectional descriptive study, 60 extracted molars with two mes...

The effect of various concentrations of thidiazuron (TDZ) with or without 2.7 μM of α-naphthalene acetic acid (NAA) on adventitious shoot formation of two Petunia hybrida cultivars was studied. Seeds from ‘Daddy Blue’ and ‘Dreams White’ cultivars were germinated in vitro. Expanded leaves from both seedlings and regenerated shoots were taken as explants. Explants were placed on MS media supplied...

Highly efficient in vitro regeneration system has been developed for Swertia lawii Burkill, an important herb used as substitute for Swertia chirayita. Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.4 ± 0.8) were obtained on MS media containing TDZ (3.0 mg l-1) in combinat...

Precociously germinating Brassica napus (oilseed rape) embryos produce extra cotyledons or chimeric organs with sectors of cotyledon and leaf tissue, rather than leaves, at the shoot apex. To investigate this phenomenon in more detail, scanning electron microscopy was used to examine the development of organ primordia at the shoot apex. In situ hybridizations with molecular markers of the embry...

Background: Stevia rebaudiana Bertoni, an important anti-diabetic medicinal plant, becomes an inevitable alternative to sugar. Due to the propagation difficulties, tissue culture is the best alternative for rapid mass propagation of stevia plants. Objective: The present study was conducted to optimize a protocol for rapid micropropagation of Stevia rebaudiana by shoot tip explants and to inv...

different explants of crocus sativus l. such as corm, style, ovary, petal, anther and leaf were cultured on ls and ms media supplemented with naa and bap or 2,4-d plus bap at different combinations. shoot and corm induction occurred in addition to the formation of other organs. efficient plantlet regeneration with strong roots occurred at 1 mg 1-i of both naa and bap. an increase in 2,4-d level...

Morphogenic potential of cabbage cv. Pride of India, for multiple shoot induction was tested under in vitro conditions using cotyledon and hypocotyl explants. Aseptically grown seven to nine days old seedlings of cabbage were used as source of explants for reproducible plant regeneration studies. Forty different concentrations and combinations of TDZ (alone), TDZ with adenine, TDZ with NAA and ...