the first successful and an efficient regeneration system on in vitro direct plant regeneration of agastache foeniculum was reported. the experiment were done in factorial design with three replications and explants were cultured in ms media fortified with different concentrations of bap and tdz (0, 2.2, 4.4, 8.8 μm) in combination with 3 levels of iaa (0, 1.1 and 2.2 μm). anova analysis showed...

Salicylic acid (SA) is one of numerous phenolic compounds found in plants with numerous important physiological events. Exogenously application of Salicylic acid (SA) is useful for the growth and development of plants. This investigation aimed to study the improving effect of salicylic acid on the tissue culture of Ziziphus spina-christi. To study its improving effect were used shoot-tip explan...

Within a plantation of clonal somatic embryo-derived white spruce trees that belonged to four genotypes, one genotype (G6) has consistently responded for the last 16 years, to the induction of somatic embryogenesis within primordial shoot explants. Analysis of fourteen individuals within this genotype subsequently revealed a group of clonal trees that were nonresponsive. This in turn provided a...

Atalantia monophylla (L.) DC. is a medium sized medicinal tree used in Ayurveda, Siddha and Traditional systems of medicine. It is used for the treatment of chronic rheumatism and paralysis, as an antidote for snake bites and shows antibacterial, antifungal and antifeedant activity. The study aims to develop a protocol for multiple shoot induction from nodal and root explants. After surface ste...

An effi cient and reproducible plant regeneration procedure is essential for introducing genes of interest in important crops through genetic transformation. However, some crops, such as soybean [Glycine max (L.) Merrill], are diffi cult to manipulate in vitro, often depending on their genotype, and the reproduction of the established protocols is not always possible. The purpose of this paper ...

An efficient in-vitro micropropagation protocol has been developed for Berberis chitria (Lindl.) - a rare medicinal plant from Himachal Pradesh, India using seeds and nodal explants. The effects of different concentrations BAP GA3 seed germination was investigated consequently 6.0 mg/l + 0.0 reported to be excellent germination. Callus induction leaf explants aseptically grown seedling were als...

Celastrus paniculatus, belonging to the family Celastraceae, is an important medicinal plant of India. Owing to the ever-increasing demand from the pharmaceutical industry, the species is being overexploited, thereby threatening its stock in the wild. Poor seed viability coupled with low germination restricts its propagation through sexual means. Thus, alternative approaches such as in vitro te...

Leaf and Cotyledon explants of Withania somnifera (L). Dunal were used to evaluate the effect of different growth regulators on the in vitro direct shoot and root initiation methods. Four different explants were used to establish callus shoot and root direct regeneration. In the first experiment leaf segments were cultured on MS basal supplemented with 2,4 – Dichlorophenoxyacetic acid (2,4 – D,...

1. Asparagus racemosus is an herb used as a rasayana in Ayurveda. The root of A racemosus is popular for its various medicinal uses such as appetizer, stomachic, tonic aphrodisiac galactogogus etc. In this experiment we have found the shoot induction after 3 weeks when Nodal explants segments were cultured in basal medium supplemented with BAP, Kin and Ads and Shoots induction on MS basal mediu...

Somatic embryogenesis has been accomplished from tender leaf base explant of date palm (Phoenix dactylifera L.). Three to five mm long tender leaf base explants derived from the meristamatic region of 2-3 year old offshoots of date palm cv. Kheneizi were cultured on Murashige and Skoog (MS) basal medium supplemented with 10, 50, 100 and 150 mg l 2,4-dichlorophenoxy acetic acid (2,4-D) and incub...