Retrovirus-mediated gene transfer is currently limited by random Brownian motion ofthe retrovirus. This limitation can be overcome by flowing the retrovirus solution through a porous membrane that supports the target cells, leading to a significant increase in the transduction efficiency. This procedure is termed "flow-through transduction." In this study, we characterized the effects of the fl...

We demonstrate the frequent accidental enrichment of spontaneous sporulation-deficient mutants of Bacillus anthracis on solid medium and identify contributing factors. Mutations in spo0A, encoding the master regulator of sporulation initiation, were found in 38 of 53 mutants. Transductions using bacteriophage CP51 propagated on sporogenic bacteria allowed for the restoration of sporulation phen...

In the present thesis we introduce several ways of extending restarting automata to devices for realizing binary (word) relations, which is mainly motivated by linguistics. In particular, we adapt the notion of input/output-relations and proper-relations to restarting automata and to parallel communicating systems of two restarting automata (PC-systems). Further, we introduce a new model, calle...

To investigate signal regulation models of gastric cancer, databases and literature were used to construct the signaling network in humans. Topological characteristics of the network were analyzed by CytoScape. After marking gastric cancer-related genes extracted from the CancerResource, GeneRIF, and COSMIC databases, the FANMOD software was used for the mining of gastric cancer-related motifs ...

Gonadotropin-releasing-hormone (GnRH) analogues are synthetic compounds derived from decapeptide neurohormones (LHRH; LH/FSH-RH). They have a key role in hormone dependent cancer, particularly breast and prostate cancer. GnRH analogues produce an efficient inhibition of gonadotropins and sex steroid hormones. Their use in cancer therapy result in a, pharmacological castration (i.e. ovariectomy ...

Introduction In formal language theory, several models characterize regular languages: finite au-tomata, congruences of finite index, monadic second-order logic (MSO), see e.g. [Büc60, Elg61, Tra61]. Further connections exist between monoid varieties and logical fragments of MSO, for instance aperiodic monoids have been shown to recognize exactly the word languages defined by first-order (FO) f...

Abstract Adaptor proteins are critical for signal transduction events involving cytokine signaling, host-response, and autoimmune diseases. Our laboratory is interested in understanding the immune regulatory role of 14-3-3zeta (z), an adaptor protein, transductions We examined 14-3-3z interleukin-17 (IL-17A) signaling inflammatory arthritis using genetically modified cells rats. The IL-17A requ...