Extracellular multi-unit recording is a widely used technique to study spontaneous and evoked neuronal activity in awake behaving animals. These recordings are done using either single-wire or multiwire electrodes such as tetrodes. In this study we have tested the ability of single-wire electrodes to discriminate activity from multiple neurons under conditions of varying noise and neuronal cell...

The time course of currents mediated by native and recombinant glycine receptors was examined with a combination of rapid agonist applications to outside-out patches and single-channel recording. The deactivation time constant of currents evoked by brief, saturating pulses of glycine is profoundly affected by the chloride concentration on the intracellular side of the cell membrane. Deactivatio...

Activity from a number of neighboring neurons can be recorded simultaneously with multichannel microelectrodes. A new version of a 24-channel microelectrode system has been fabricated and used to record different types of neurophysiological data in the rat brain. The system called PRONG (Parallel Recording Of Neural Groups) includes a microelectrode, a lightweight reusable connector, a 24-chann...

Using single-channel recording, we show that neurons contain ion channels sensitive to membrane tension. Neurons isolated from the snail, Lymnaea stagnalis, actively rearborized in culture yielding cell bodies and growth cones suitable for patch clamping. All neurons contained, in both their soma and growth cones (at a density of approximately 1-2 micron-2), stretch-activated channels highly se...

A NEURAL RECORDING FRONT END FOR MULTI-CHANNEL WIRELESS IMPLANTABLE APPLICATIONS By Haitao Li By recording neurons’ activities simultaneously, awake animal behaviors can be predicted in real time, brain–machine interfaces controlling the machine by thought can be set up, and treatments for neurological disorders can be explored. Existing commercial neural recording equipment are bench-top syste...

introduction: we have recently reported the presence of two potassium currents with 598 and 368 ps conductance in the rough endoplasmic reticulum (rer) membrane. the 598 ps channel was voltage dependent and atp sensitive. however, the 368 ps channel was rarely observed and its identity remained obscure. since cationic channels in intracellular organelles such as mitochondria and rer play import...