Infectious bursal disease virus of chickens was purified, and its structure was examined by the negative-staining technique in the electron microscope. The buoyant density of infectious bursal disease virus in CsCl was found to be 1.34 g/cm(3). The morphological details suggest that the capsid of the virion consists of a single layer of 32 capsomeres arranged in 5:3:2 symmetry. The virion measu...

Bacteriophage N4 virion RNA polymerase transcription of double-stranded promoter-containing DNAs requires supercoiled template and E. coli single-stranded DNA-binding protein (EcoSSB); other single-stranded DNA-binding proteins cannot substitute. The DNA determinants of virion RNA polymerase binding at the promoter comprise a small template-strand hairpin. The requirement for EcoSSB is surprisi...

The aim of the present study was to examine the effects of scriptaid (SCR), a histone deacetylase inhibitor (HDACi), on the in vitro and in vivo development of Wuzhishan miniature pig somatic cell nuclear transfer (SCNT) embryos. Experiment 1 compared the in vitro developmental competence of nuclear transfer embryos treated with various concentrations of SCR for 24 h. Embryos treated with 250 n...

HIV-1 infectivity is achieved through virion maturation. Virus particles undergo structural changes via cleavage of the Gag polyprotein mediated by viral protease, causing transition from an uninfectious to infectious status. The majority proviruses in people living with treated combination antiretroviral therapy are defective large internal deletions. Defective proviral DNA frequently preserve...

In this study, we establish that cholesterol and sphingolipid associated with hepatitis C virus (HCV) particles are important for virion maturation and infectivity. In a recently developed culture system enabling study of the complete life cycle of HCV, mature virions were enriched with cholesterol as assessed by the molar ratio of cholesterol to phospholipid in virion and cell membranes. Deple...

Since the Euro-III standard was adopted, the main methods to inhibit NOx production in diesel engines are exhaust gas recirculation (EGR) and select catalyst reduction (SCR). On these methods SCR offers great fuel economy, so it has received wide attention. However, there also exists a trade-off law between NOx conversion efficiency and NH3 slip under dynamic conditions. To inhibit NH3 slip wit...

ÐWriting requirements in a formal notation permits automatic assessment of such properties as ambiguity, consistency, and completeness. However, verifying that the properties expressed in requirements are preserved in other software life cycle artifacts remains difficult. The existing techniques either require substantial manual effort and skill or suffer from exponential explosion of the numbe...

BACKGROUND The performance of estimated glomerular filtration rate (eGFR) have been proved to vary according to the races of the target population. The eGFR equations have not been validated in the Chinese cancer population received chemotherapy. Meanwhile, serum cystatin C (CysC), urea, β2 microglobulin (β2-MG), and creatinine (SCr) were also evaluated in a cohort of Chinese cancer patients. M...

Fleet fuel consumption is greatly reduced through the introduction of the HSDI Diesel engine. The reduced fuel consumption is then reflected in a reduction of CO2 emissions. The drop in fuel consumption and CO2 emissions results in a rise in market acceptance, which is also the result of desirable driving performance and greatly improved NVH behavior. The continuously increasing demands on plac...