BACKGROUND Hydrophobic interaction chromatography (HIC) most commonly requires experimental determination (i.e., scouting) in order to select an optimal chromatographic medium for purifying a given target protein. Neither a two-step purification of untagged green fluorescent protein (GFP) from crude bacterial lysate using sequential HIC and size exclusion chromatography (SEC), nor HIC column sc...

A new thermal field-flow fractionation (ThFFF) method has been developed for the separation and analysis of polyacrylates and acrylic-styrene copolymers. This important class of polymers is commonly used as pressure sensitive adhesives, in coatings and paintings, and as the basis for polyelectrolyte materials. The structure and chemical composition of these polymers play a vital role in the end...

A liquid chromatography (HPLC) method with UV detection was developed for determination of sodium hyaluronate in pharmaceutical formulation. Sodium hyaluronate is a polymer of disaccharides, composed of d-glucuronic acid and d-N-acetylglucosamine, linked via alternating β-1, 4 and β-1, 3 glycosidic bonds. Being a polymer compound it lacks a UV absorbing chromophore. In the absence of a UV absor...

Heterogeneity is a fact that plagues the characterization and application of many self-assembled biological constructs. The importance of obtaining particle homogeneity in biological assemblies is a critical goal, as bulk analysis tools often require identical species for reliable interpretation of the results-indeed, important tools of analysis such as x-ray diffraction typically require over ...

Nanodiscs are membrane mimetics which may be used as tools for biochemical and biophysical studies of a variety of membrane proteins. These nanoscale structures are composed of a phospholipid bilayer held together by an amphipathic membrane scaffold protein (MSP). In the past, nanodiscs were successfully assembled with membrane scaffold protein 1D1 and 1,2-dipalmitoyl-sn-glycero-3-phosphorylcho...

Characterization of the molar mass distribution (MMD) poly (ether ether ketone) (PEEK) is very troublesome due to insolubility PEEK in common solvents. The solubility may be substantially improved after its sulfonation. Sulfonated (SPEEK) was prepared by sulfonation with 98% concentrated H2SO4 at room temperature. presence sulfonic acid group (-SO3H) matrix confirmed nuclear magnetic resonance ...

At the start of Archie Hamielec's research career, size exclusion chromatography (SEC) (including gel permeation [GPC]) was in its infancy as an analytical and preparative laboratory technique polymer science engineering. As described below, Hamielec saw potential early his academic career among group pioneers who took it from a developing to what is now. His group's work instrumental improving...

Field-flow fractionation (FFF) is a powerful alternative to column-based polymer fractionation methods such as size-exclusion chromatography (SEC) or interaction chromatography (IC). The most common polymer fractionation method, SEC, has its limitations when polymers with very high molar masses or complex structures must be analysed. Another limitation of all column-based methods is that the sa...

Cellulosic bottlebrushes with polystyrene (PS) and poly(ethylene glycol) (PEG) side chains at the O-6 O-2,3 positions, respectively (PEG-PS-cellulose), were synthesized characterized in diluted solution to reveal second structure of heterografted bottlebrushes. The regioselectivity degree substitution evaluated by 1H- 13C-nuclear magnetic resonance spectroscopy size-exclusion chromatography (SE...