Deoxynivalenol (DON) commonly infects agricultural foods; it exhibits toxicity by inducing oxidative stress and inhibiting protein synthesis. Nuclear factor erythroid 2-related 2 (NRF2) regulates the cellular antioxidant response. We investigated cytotoxicity of DON its effect on NRF2 response in HepG2 cells. The Methyl Thiazol Tetrazolium (MTT), glutathione (GSH) ATP assays evaluated toxicity,...

Abstract Introduction Ischaemia reperfusion injury (IRI) is an unavoidable, significant consequence of renal transplantation. MicroRNAs are small, non-coding RNA molecules that regulate multiple downstream mRNA targets. MiRNA-21-5p and miRNA-24-3p have been previously implicated in IRI. Antisense oligonucleotides (ASOs) block specific microRNAs, with previous work by our group demonstrating the...

Hyperglycemia in Diabetes mellitus (DM) induces oxidative stress and mitochondrial disruptions the liver, thereby triggering apoptosis through Bcl-2 family signaling. Excessive leads to liver dysfunction. Chlorogenic acid (CGA) was elucidated this study preventing dysfunction as a progression of DM. The diabetic model conducted Wistar rats, divided into six groups. Blood examinations were done ...

Vitrification of preantral follicles is a promising technique to preserve female fertility. The aim the present study was evaluate effect supplementation α-tocopherol in vitrification solution on viability, lipid peroxidation and mRNA expression superoxide dismutases (SOD1 SOD2) vitrified cultured ovine at day-6 day-12. Preantral (200-300 µm) were isolated from ovaries by mechanical method dist...

OBJECTIVE Transforming growth factor-β-activated kinase 1 (TAK1) is a mitogen-activated protein 3-kinase and an AMP-activated protein kinase (AMPK) kinase in some cell types. Although TAK1(-/-) mice display defects in developmental vasculogenesis, the role of TAK1 in endothelial cells has not been investigated in detail. APPROACH AND RESULTS TAK1 downregulation (small interfering RNA) in huma...

A Bax- and, apparently, mitochondria-dependent increase in superoxide (O(2)(·-)) and other reactive oxygen species (ROS) occurs in apoptotic superior cervical ganglion (SCG) and cerebellar granule (CG) neurons. Here we show that Bax also lies upstream of ROS produced in nonapoptotic neurons and present evidence that caspases partially mediate the pro-oxidant effect of Bax. We used the O(2)(·-)-...

Oxidative stress is significant in numerous types of cancer. Tobacco smoke, an important risk factor for oral squamous cell carcinoma (OSCC), is able to generate reactive oxygen species (ROS) and cause oxidative DNA damage. Superoxide dismutase (SOD) is an endogenous antioxidant enzyme that is critical in limiting the oxidative burden effectively. The purpose of this study was to investigate th...