Sodium dodecyl sulfate (SDS) is a strong surfactant that is widely used in protein sample preparation. While protein and peptide samples containing up to approximately 1% SDS can be analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) using a two-layer matrix/sample deposition method, the presence of SDS in a protein sample generally degrades mass resolution and ...

We describe a procedure for purification of creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) from human cardiac muscle by preparative electrophoresis on polyacrylamide gel. From a 50-g portion of human myocardium we isolated 21 mg of CK-MB, which had a specific creatine kinase activity of 405 kU/g. The CK-MB exhibited an enzyme band on polyacrylamide gel electrophoresis (PAGE) with staining fo...

Cyclooxygenase-2 (COX-2) Renal mesangial cells (RMC) Wild type (WT) Adenovirus (Ad) Tumour necrosis factor α (TNFα) Endothelin-1 (ET-1) Interleukin 1β (IL-1β) Prostaglandin E2 and prostaglandin I2 (PGE2 and PGI2) Annexin V (AV) Fluorescein isothiocyanate (FITC) Propidium iodide (PI) Viable (V), early membrane intact apoptotic (EA) and necrotic (N) Hanks Buffered Saline Solution (HBBS) Phosphate...

One component of human lymphoblastoid interferon obtained from Namalwa cultures induced by Newcastle disease virus has been purified to a specific activity of 2.5 x 10(8) interferon units per mg of protein (protein content based on amino acid analysis). A single polypeptide species with an apparent molecular weight of 18,500 comigrating with the antiviral activity was observed by sodium dodecyl...

A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4'-azobenzene...

Serum samples from patients with various forms of rubella virus infection were tested for antibodies to each of three viral structural proteins by radioimmunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In most sera antibody to E1 protein was the predominant species. Sera from patients with congenital rubella syndrome, however, contained significantly more E2 ant...

Pseudomonas aeruginosa K/2PfS, when transformed with an expression plasmid harboring the pilin gene (pilE1) of Neisseria gonorrhoeae MS11, was able to express and assemble gonococcal pilin monomers into surface-associated pili, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and immunoelectron microscopy. Concomitant with the expression of gonococcal pili...

Recombinant human leukocyte interferon produced in bacteria (IFLrA) was purified to homogeneity with the use of monoclonal antibodies against leukocyte interferon. The purified interferon exhibited a single band of Mr = approximately 19,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Amino acid analysis and the NH2-terminal sequence were consistent with the sequence predicted ...

A total of 1,417 staphylococcal and micrococcal strains were collected from the beards and scalps of 10 subjects over a period of 8 months. Sixteen strains identified as Staphylococcus epidermidis with an API system had distinctive yellow colonies on nutrient agar plates and sodium dodecyl sulfate-polyacrylamide gel electrophoresis whole-cell polypeptide profiles similar to those of Staphylococ...

The heterogeneity of the surface material released by trypsin in isolated cells of melanotic and amelanotic melanomas was studied by the method of separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The electrophoretic patterns of the surface material derived from two kinds of melanoma showed some differences. The differences in the surface proteins and glycoproteins seem t...