We report here the first case of an affinity isolation of eukaryotic RNA polymerase II. The procedure employs an affinity matrix composed of alpha-amanitin coupled to Sepharose 4B via a ten atom spacer. RNA polymerase II from either calf thymus or wheat germ binds to the amanitin-Sepharose, as indicated by subsequent elution with sodium dodecylsulfate-containing buffer and analysis by polyacryl...

In this research, the influence of additives on rheological and sedimentary properties magnetorheological fluid (MRF) was tested analyzed. The were stearic acid, sodium dodecyl sulfate (SDS), their mixture, respectively. MRF composed carbonyl iron particle, silicone oil, liquid paraffin, graphite bentonite, SDS. results indicated that mainly influenced by mass fraction particle. When fractions ...

Alpha-benzilmonoxime in sodium dodecylsulfate micellar media has been used for the spectrophotometric determination of cobalt at pH 9.0. The linear range of calibration is 0.05-1.50 microg cm(-3) of cobalt at 380 nm with molar absorptivity of 3.72 x 10(4) dm3 mol(-1) cm(-1), which is about 1.5-times greater than that of the alpha-benzilmonoxime extraction based method. The relative standard dev...

Plasma electrolytic oxidation (PEO) coatings on AZ91 Mg alloy were sealed in calcium nitrate solutions at different pH values (3, 4 and 5). Sodium dodecyl sulfate (SDS) was added to study the effect of SDS corrosion resistance coatings. The microstructure, chemical composition investigated by means SEM, XPS, XRD electrochemical tests. performance improved due precipitation Ca-containing precipi...

Prediction of Surfactants’ Properties using Multiscale Molecular Modeling Tools: A Review — During one of the existing Enhanced Oil Recovery (EOR) procedures, a mixture of Alkaline/Surfactant/Polymer (ASP) is injected into wells in order to move the trapped oil from the reservoir to the wellbores. The conception and/or the tuning of new ASP combinations, structures of surfactants and/or mixture...

the anionic surfactant sodium dodecyl sulfate (sds) was degraded by novel strain ofpseudomonas aeruginosa kgs under accession no. jq328193, which was isolated from carwash wastewater. the purpose of this research was to study different optimization conditionsrequired for enhancing the biodegradation of sodium dodecyl sulfate p. aeruginosa kgs.influence of different physicochemical factors such ...

sodium oxalate without SDS) by varying the concentration of the dye (0.03 or 0.12 mmol/L), sodium oxalate (0 or 2 mmol/L), and SDS (25 or 100 mg/L) in all combinations. For protein assays, we mixed 20 ␮L of sample, calibrator, or water (blank) with 1 mL of PRM reagent and measured the absorbance at 600 nm after 30 min against the blank. The effects of the aminoglycosides on the absorbance readi...

Sodium dodecyl sulfate (SDS)-poly(propylene oxide) methacrylate (PPOMA) (of molecular weight M(w) = 434 g x mol(-1)) mixtures have been studied using conductimetry, static light scattering, fluorescence spectroscopy, and 1H NMR. It has been shown that SDS and PPOMA form mixed micelles, and SDS and PPOMA aggregation numbers, N(ag SDS) and N(ag PPOMA), have been determined. Total aggregation numb...