Cyanide binding to bovine heart cytochrome c oxidase at five redox levels has been investigated by use of infrared and visible-Soret spectra. A C-N stretch band permits identification of the metal ion to which the CN- is bound and the oxidation state of the metal. Non-intrinsic Cu, if present, is detected as a cyanide complex. Bands can be assigned to Cu+CN at 2093 cm-1, Cu2+CN at 2151 or 2165 ...

The phenomenon of convective flow with heat and mass transfer has been studied extensively due to its applications in various fields. effects nonlinear thermal radiation (NLTR), slip, thermal-diffusion (Soret) diffusion-thermo (Dufour) on magenoto-hydrodynamic (MHD) towards a stretching cylinder the presence triple stratification (TSF) are investigated this paper. governing equations transforme...

Interaction of the two high-spin hemes in the oxygen reduction site of the bd-type quinol oxidase from Escherichia coli has been studied by femtosecond multicolor transient absorption spectroscopy. The previously unidentified Soret band of ferrous heme b(595) was determined to be centered around 440 nm by selective excitation of the fully reduced unliganded or CO-bound cytochrome bd in the alph...

1. It is shown that free haematins will react with nitric oxide under certain conditions giving compounds with characteristic absorption spectra. 2. The reactions of nitric oxide with both protohaematin and urohaematin were examined. In acid solution each of these haematins changes colour from reddish brown to a bright red in the presence of nitric oxide, and two strong absorption bands appear ...

By mutant colony screening of Caldariomyces fumago a mutant was isolated which was slightly greenish on fructose minimal medium and grew slower in comparison to the wild type. The supernatant samples lacked the Soret band typical for the heme group of the CPO and nearly no CPO activity was detected. SDS-PAGE analysis of mutant culture supernatant samples showed production of a 38-40 kDa protein...

Photodynamic therapy is an advanced method of treating cancer and various benign diseases, including infections. It uses light-activated molecules [photosensitizers (PSs)] to generate reactive oxygen species (ROS) when irradiated with light of a specific wavelength. This study examined the photophysical and photosensitizing activity of the PS chlorin e6 incorporated in a delivery system based o...

The first and second generation of zinc porphyrin core dendrimers (3 and 4) with triarylamine as dendron have been synthesized via Ullmann coupling reaction. Their absorption and emission spectra indicate that there are strong interactions between zinc porphyrin core and triarylamine dendrons. Zinc porphyrin links with triarylamine causes Soret band broadening and Q band shift as compared with ...

Ferricytochrome c showed low-level chemiluminescence, with a light-emission measured of about 1x10(3)-3x10(3) counts/s, when supplemented with organic hydroperoxides. Tertiary hydroperoxides (cumene hydroperoxide and t-butyl hydroperoxide) showed a saturation behaviour at about 5mm-hydroperoxide, whereas primary hydroperoxides showed a quadratic dependence on the hydroperoxide concentration. Ch...

The optical spectrum of reduced myeloperoxidase (EC 1.11.1.7) displays an unusual red shift of the Soret band which is at 472 nm and the alpha-band which is at 636 nm. The spectral properties of myeloperoxidase can be modified by means of acid treatment. Upon short exposure to acid (pH 1.7) the red-shifted optical absorption spectrum of the reduced enzyme (lambda max at 472 nm) was blue-shifted...

The sulfhydryl titration of hemoglobin Rainier (wl32 146Tyr-‘Cys) suggests that the cysteine residue HC2(145)/3 forms a disulfide bridge with the cysteine F9(93)/3 of the same /3 chain. The oxygen equilibrium of Hb-Rainier compared with that of HbA exhibits diminished heme-heme interaction (n = 1.3-1.5), increased oxygen affinity, and a small Bohr effect. The absorption spectrum and the circula...