There are many reports on the appearance of a typical isoenzyme of alkaline phosphatase (EC 3.1.3.1; API)2 in serum of patients suffering from biliary obstruction. According to the characteristics observed in various studies, this API has variouslybeen termed “high molecular weight” (1), “origin,” “stationary,” “particulate” (2), “bile,” “cholestatic,” “pathological biliary” (3, 4), and “koinoz...

Rheumatic carditis is the most serious major manifestation of acute rheumatic fever in children. Cardiac Troponin-T (cTnT) is established as a new specific marker of myocardial damage or injury. The present work was carried out to study the value of cTnT as a diagnostic marker of myocardial injury in children with rheumatic carditis, and to compare it to established parameters of myocardial inj...

Perinatal hypoxia is one of the more common complications early adaptation period. This condition defined as a disorder tissue oxygenation during labour and responsible for approximately 23% neonatal mortality. can cause injury failure many organs, including brain, heart kidneys, mainly in mechanism ischaemic-reperfusion injury. Currently, biochemical markers are frequently incorporated diagnos...

treatment with PLA,. At this stage it is impossible to say whether the presence of such large amounts of lysophospholipids has a role in isoenzyme inactivation. Nevertheless it can be hypothesized that each P450 isoenzyme responds differently to changes in membrane NEFA content and composition. This situation might facilitate the selective modulation of P450 isoenzyme activity depending on the ...

We used an RIA and inhibition of enzyme activity to monitor the changes in mass and catalytic concentrations of the aspartate aminotransferase (EC 2.6.1.1;AST) isoenzymes in serum after myocardial infarction. Cytosolic (c-AST) and mitochondrial (m-AST) forms of AST were present in sera from all 38 of our patients. Although the immunological and catalytic concentrations of both isoenzymes correl...

The creatine kinase (CK) isoenzyme system is essential for motility in rooster and sea urchin sperm. In the present study, biochemical characterization as well as immunofluorescence and confocal laser microscopy with highly specific antibodies against various chicken CK isoenzymes revealed that cytosolic brain-type CK isoenzyme (B-CK) is the only CK isoenzyme in rooster seminal plasma, while th...

Isoenzyme-based studies have identified 3 taxa/species/'phylogenetic complexes' as agents of visceral leishmaniasis in Sudan: L. donovani, L. infantum and "L. archibaldi". However, these observations remain controversial. A new chitinase gene phylogeny was constructed in which stocks of all 3 putative species isolated in Sudan formed a monophyletic clade. In order to construct a more robust cla...

-DEoxY-D-arabino-heptulosonate 7-phosphate synthase (DAHP synthase) is 3 the first enzymic function specific to aromatic biosynthesis. In Neurospora crassa, there are three DAHP synthase isoenzymes, each subject to feedback inhibition by one of the three aromatic amino acids (DOY 1968, 1970; JENSEN and NASSER 1968) : phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp) . Mutations inactiva...

Activity of serum/plasma beta-N-acetylhexosaminidase (EC 3.2.1.52) was determined by means of a continuous spectrophotometric method using 3,3'-dichlorophenylsulphonphthaleinyl-N-acetyl-beta-D-glucosaminid e as substrate, with very satisfactory results. Incubation of an undiluted aliquot (1 ml) of samples at 52 degrees C for 8 hours with an adjusted pH 5.5-6.0 provoked only the inactivation of ...

We examined the effect of storing human plasma or extracts of prostate at -90 degrees C on the activity of creatine kinase and lactate dehydrogenase and isoenzyme distribution. Enzyme activities were unaltered during storage for as long as six weeks. If these preparations were thawed only once at 2 to 4 degrees C, they could be stored for as long as 165 days at -90 degrees C with no change in i...