BACKGROUND Measurement of complex I activity has been hampered by the large amounts of tissue required and the resulting turbidity of the assay solution, which makes spectrophotometric analysis difficult. We have developed a new assay for measuring the activity of complex I in isolated mitochondria that is also applicable to skeletal muscle homogenate in patients with suspected mitochondrial di...

In the past two decades public interest in herbal products has increased significantly in Europe, especially in the plant-based products from non-European traditions. Eleutherococcus senticosus has been used for the treatment of inflammatory diseases, anemia, and rheumatoid arthritis. The Eleutherococcus senticosus fruits intractum was examined for the content of phenolic acids (LC-ESI-MS/MS), ...

The kinetic properties and substrate specificity of two well-characterized peptidyl prolyl cis-trans isomerases (PPIases), cyclophilin and the FK-506 binding protein (FKBP), have been previously examined [Fischer, G., Bang, H., Berger, E., & Schellenberger, A. (1984) Biochim. Biophys. Acta 791, 87-97; Harrison, R.K., & Stein, R.L. (1990) Biochemistry 29, 1684-1689; Albers, M.W., Walsh, C.T., & ...

dehydrogenases. NAD+ and NADP+ are reducible at the dropping mercury electrode, and the cathodic waves may be measured in the region -1.1 V to -1.3 V. The reduced nucleotides NADH and NADPH are not electroactive at these potentials and thus do not interfere with themeasurements. Under somecircumstances, this polarographic assay for a dehydrogenase may offer advantages over the conventional spec...

Osmotic drying of celery leaves was studied in two osmotic solutions (ternary aqueous solution and sugar beet molasses), at three temperatures (20, 35, 50?C), diverse immersion periods (1, 3, 5 hours). The aim to examine the influence used hypertonic agent, temperature time on antioxidant capacity (AOC) color characteristics samples. AOC assessed by spectrophotometric assays (ABTS, FRAP, DPPH),...