Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evalua...

The research aimed to determine the effect of different taurine concentrations on duration sperm fertility preservation and results further use during cryopreservation. was injected into Siberian sturgeon by various methods under conditions low positive temperatures. Immediately after preparation, native at 0.01, 0.05, 0.1 mmol/ml. Two introducing were tested: 1 (dry method) - adding powder dir...

There is growing concern over the effect of sperm cryopreservation on DNA integrity and the subsequent development of offspring generated from this cryopreserved material. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure t...

Spermatozoa cryopreservation is an effective method for maintaining male fertility in humans. Nevertheless, there are some limitations of sperm cryopreservation, which called as cell injury by cryoprotectant, that cannot be avoided. This process will affect embryo quality. Therefore, it mandatory to modify cryoprotectant spermatozoa improve review aimed summarize the modification can damage cel...

Sperm cryopreservation is a method to preserve sperm samples for a long period. However, the fertility of sperm decreases markedly after freezing and thawing in a certain amount of samples. The aim of the present study was to find useful and reliable predictive biomarkers of the capacity to withstand the freeze-thawing process in human ejaculates. Previous researches have shown that enolase1 (E...

BACKGROUND This was a retrospective study to determine if diagnostic testicular biopsy followed by cryopreservation should be the procedure of choice for all patients with testicular failure. METHODS The first part of the study analysed 97 ICSI cycles scheduled with frozen-thawed testicular sperm for 69 non-obstructive azoospermia (NOA) patients. The second part focused on a subgroup of 32 pa...

While the idea of freezing human male gametes has been experimented since the late 1700's, it was not until 50 years ago that human sperm were frozen, and later thawed in such a way that they could fertilize an egg and initiate development. In the past several decades the technology of cryopreservation, or maintaining life in a frozen state, has advanced considerably. With the use of modern tec...

Abstract Fertilization was doing to measure the success of sperm cryopreservation because it more informative. Sperm expected get that successfully fertilize egg well. The aim this study determine fertilization ability giant grouper (Epinephelus lanceolatus, Bloch 1790) 48 hours post-cryopreservation in fertilizing tiger eggs fuscoguttatus, Forskal 1775). carried out artificially using with 6% ...

The extracellular matrix is a poorly studied, yet important component of dental biofilms. Fluorescence lectin-binding analysis (FLBA) is a powerful tool to characterize glycoconjugates in the biofilm matrix. This study aimed to systematically investigate the ability of 75 fluorescently labeled lectins to visualize and quantify extracellular glycoconjugates in dental biofilms. Lectin binding was...

introduction: during early neural tube formation, the notochord is essential for the induction of ectoderm and for the subsequent differentiation of the neuroepithelium forming diverse cell types in the neural tube. due to the key role of the glycoconjugates in many developmental events, the early distribution of these molecules in early notochordal interaction with adjacent tissues were studie...