Strategies to preserve fertility in dogs and cats as cryopreservation of epididymal spermatozoa, oocytes and ovarian tissue will be discussed in this review. Recent results indicate that significant progress have been achieved, but optimal freezing protocols have not been defined yet. The extreme sensitivity of sperm membranes and of oocyte and ovarian tissue structure deserves further investig...

background: cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. the main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. recently, the application of controlled mild stressors has been described for activation of a temporary response in oocy...

The aim of this study was to compare the effect of different packaging systems on some parameters of cryopreserved canine spermatozoa. The experimental material consisted of the sperm-rich fractions of ejaculates collected from four Beagle dogs. Semen samples for cryopreservation were stored in 0.25 ml plastic straws and two aluminum tubes with a total volume of 5.0 ml. Semen was frozen in stat...

This study aimed to analyze the effect of Alpinia purpurata K. Schum rhizome extract (APRE) supplementation into tris-egg yolk extender on sperm quality Bali bull after freezing. used experimental method with a completely randomized design (CRD), consisting five treatments, denoted as P0 (semen diluted using 100% Tris-Egg Yolk (TEY)), P1 98% TEY and 2% APRE), P2 96% + 4% P3 94% 6% P4 92% 8% APR...

Bull spermatozoa were pellet frozen on dry ice in a TES buffer containing combinations of egg yolk and glycerol to determine which component of an extender afforded cryoprotection to the spermatozoa. Neither buffer nor buffer plus glycerol protected sperm cell motility in the absence of egg yolk, while sperm cells frozen in egg yolk buffer in the absence of glycerol had 24% postthaw motility (P...

This experiment was designed to compare powdered egg yolk with fresh egg yolk in an extender for cryopreservation of Zebu bull semen. Sperm motility, plasma membrane integrity and viability were assessed at different stages of cryopreservation (post-dilution, pre-freezing and post-thawing). Sperm plasma membrane integrity remained similar at all the stages of cryopreservation. Sperm motility an...

Background Cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. The main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. Recently, the application of controlled mild stressors has been described for activation of a temporary response in oocyt...

Oxidative stress is an important component of the cytopathology of equine spermatozoa undergoing storage as liquid or frozen semen. Damage to chromatin, membranes and proteins of sperm are important components of oxidative damage to sperm. Similarly, sperm are exposed to a variety of osmotic stresses during storage that result from exposure to hypertonic media or result as a consequence of osmo...

It is universally recognized that cryopreservation impairs sperm quality. In order to improve postthawing sperm survival and motility, media of different composition and different protocols have been proposed. However, no clear evidence is available to understand which are the most efficient protocol and medium for sperm cryopreservation. The present study evaluates the efficiency of two differ...

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...