formalin is widely used in industry and in medicine (as tissue fixative and disinfectant).it contains reactive molecules which have been known for its cytotoxic effects. to evaluate the effect of formalin exposure on the testicular tissue and sperm parameter from neonatal period through physical and sexual maturity, 28 male wister rats were assigned into two equal test and control groups. the t...

Cytological and autoradiographic studies were done to investigate the cytoplasmic control of DNA synthesis under conditions of physiological polyspermy. The DNA synthetic phases of the egg, principal sperm and accessory sperm nucleic were determined and correlated with nuclear morphology and develpmental fate. Results show that accessory sperm nuclei undergo morphological transition to pronucle...

BACKGROUND The present study aimed to evaluate the efficacy of the hyaluronic acid (HA) binding assay in the selection of motile spermatozoa with normal morphology at high magnification (8400x). METHODS A total of 16592 prepared spermatozoa were selected and classified into two groups: Group I, spermatozoa which presented their head attached to an HA substance (HA-bound sperm), and Group II, ...

Objective(s): Nicotine as a toxic substance leads to impairment of the reproductive system function. The aim of this study was to evaluate effects of melatonin on testicular alterations, sperm nuclear integrity, and epididymal sperm parameters in mice treated with nicotine.Materials and Methods: Male mice were divided into four groups. Group A received the vehicle, group B received nicotine 0.1...

Background: The fertilizing sperm potential is directly related to its mitochondria activity evaluated by measuring the mitochondrial membrane potential (ΔΨm). The objectives of the present study were to investigate the vitality and mitochondrial membrane potential of sperm in infertile men by the and to assess the correlations between sperm parameters and the level of sperm mitochondrial membr...

BACKGROUND Sperm ion channel proteins (CatSpers) are essential for sperm hyperactivated motility, and then penetration through the zona pellucida. The CatSper class of proteins have well been characterized in the mouse and human. However, such data for pigs are not available. In the present study, we cloned the porcine CatSper 1-4 genes, analysed their spatial expression in various organs and t...

Although the pathophysiology of testicular damage associated with varicocele remains unclear, sperm DNA has been identified as a potential explanation for this cause male infertility. The current study was designed to determine extent nuclear in patients varicocele, and examine its relationship parameters seminal motility.

The cell wall secreted immediately following sperm entry into an egg can be visualized by the fluorescent dye Calcofluor white. Cell wall secretion precedes nuclear fusion by 10-20 min. SEM observations of the surface of unfertilized and fertilized eggs and sperm attachment to eggs are described. These results are discussed in relation to fertilization in sea urchins and the biochemical phenome...

BACKGROUND In humans, it is now well documented that rising paternal age is correlated with decreased sperm DNA integrity and embryonic developmental failures. On the other side of the coin, it is also reported that very young fathers such as teenagers carry an increased risk of adverse birth outcomes. These observations suggest that, at least in humans, there is an age window for optimal sperm...