YES of the dye or enzyme to the sites of damaged DNA) 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 TESTS of sperm DNA integrity have been used increasingly in the evaluation of the infertile man. These tests have been developed to further our understanding of spermatogenesis and sperm function, and to...

BACKGROUND A large proportion of patients undergoing ICSI have been shown to have an increased sperm aneuploidy rate. This study was undertaken to evaluate the impact of sperm aneuploidy on ICSI outcome. METHODS To accomplish this, 48 consecutive unselected male patients (median age 34 years) had their sperm aneuploidy rate evaluated in the same swim-up preparation used for ICSI. Chromosomes ...

The mammalian estrogen induced oviductal glycoprotein (OGP) has been known to associate with capacitated sperm, oocytes and developing embryos. This study aimed to identify the putative binding partner of OGP on gametes using N-terminal peptide of bonnet monkey (Macaca radiata) OGP, Nmon, as bait. A protein(s) of molecular size ~54 kDa was detected by farwestern blot analysis of detergent solub...

Experiments were performed on 75 ejaculates obtained from 19 bulls representing different cattle breeds used at the Masovian Centre for Animal Breeding and Reproduction in Łowicz. Fresh ejaculates were measured in respect to their volume and sperm count in the ejaculates was determined. The ejaculates were classified based on the criterion of sperm concentration and divided into five groups. Sp...

Accidental pollution incidents are common in the marine environment and are often caused by oil-related activities. Here the potential of such an incident to disrupt reproduction in two polychaete species is investigated, using an environmentally relevant preparation of weathered Forties crude oil, i.e. the water accommodated fraction (WAF). Oocytes were collected and exposed to three concentra...

This paper describes an unusual association of aspermia and untreatable, chronic testicular pain in a young man who underwent 14 surgical interventions for an imperforate anus. Physical examination and ultrasonography revealed left epididymal and vas enlargement, normal-sized testes, tubular ectasia of the left rete testis and a small intraprostatic paramedian left cyst. Retrograde ejaculation ...

In April 2016 ZIKA virus RNA was detected during control before performing in vitro fertilization in adult-man semen that contracted the infection in French Guyana and travel back to France. The infection had been detectable in the sperm by qRT-PCR and the virus persists in the semen even after routinely ART sperm migration performed in a bilayer gradient. Zika virus (ZIKV) is an arbovirus tran...

sting by E Abstract Introduction: Intrauterine insemination (IUI) is a valid treatment for infertility with a cumulative pregnancy rate of 40–90% after 3–10 treatment cycles. Design: We prospectively studied the efficacy of a new simplified method for motile sperm preparation for IUI for both male causes of infertility, and for those diagnosed with idiopathic infertility. Methods: A prospective...

The preparation of sexed semen is based on the differential DNA content between the X and Y chromosome bearing sperm cells determined by fluorescence-activated cell sorting. In spite of its intrinsic limitations this represents the only effective method. However, the employment of sexed sperm for breeding food producing animals on a large scale requires additional knowledge in the protein reper...