Koonjaenak S. 2006. Semen and Sperm Characteristics of Swamp Buffalo (Bubalus bubalis) Bulls for Artificial Insemination in Thailand, in Relation to Season. Doctoral thesis. ISSN 1652-6880, ISBN 91-576-7263-6 In this thesis work we tested the hypothesis that tropical climatic conditions affect the quality of fresh and frozen-thawed (FT) spermatozoa from swamp buffalo bulls used for artificial i...

Data obtained by the sperm chromatin structure assay (SCSA) on spermatozoa from nine bulls were correlated with fertility, measured by heterospermic performance (-0.94, P less than 0.01) and by alternate tests of sperm quality, including motility, acrosome integrity, Sephadex filtration and morphology of spermatozoa (all significant at P less than 0.05 to P less than 0.01). The SCSA uses flow c...

Centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system (TPS) is a resolute technique revealing sperm heterogeneity and for the estimation of the fertilizing potential of a given semen sample. However, separated sperm subpopulations have never been tested for their fertilizing ability yet. Here, we have compared sperm quality parameters and the fertilizing ability of sperm...

This study was conducted to determine the number of spermatozoa residing in the oviduct sperm-storage tubules (SST) and the relationship between these numbers and the number of spermatozoa embedded in the perivitelline layer of oviductal eggs after a single insemination of 200 x 10(6) spermatozoa. The SST of hens inseminated within one week before the expected onset of egg production were fille...

This study compared a number of semen parameters of two separate groups of the common marmoset monkey (Callithrix jacchus) in order to determine the effect of a continuous potentially stressful situation on these parameters, and thus on the monkey's reproductive ability. The semen from 16 adult male marmoset monkeys was collected and analysed to compare semen parameters between a 'normal' (cont...

background: it is established that sperm dna integrity is essential in fertilization and normal embryo and fetal development. routine semen analysis gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm dna. therefore, the evaluation of sperm dna integrity, in addition to routine sperm parameters, could add further informat...

BACKGROUND Good quality spermatozoa are important to achieve fertilization, viable embryos and offspring. Single Layer Centrifugation (SLC) through a colloid (Androcoll-P) selects good quality spermatozoa. However, it has not been established previously whether porcine spermatozoa selected by this method maintain their fertility. METHODS The semen was prepared either by SLC or by standard cen...

The aim of this study was to identify different motile sperm subpopulations in ejaculates from an autochthonous bull breed (Bos taurus) and to determine possible modifications in these subpopulations resulting from cryopreservation. Ejaculates were collected and cryopreserved following a conventional protocol. The overall sperm motility and the kinematic parameters of individual spermatozoa wer...

Utilization of cauda epididymis spermatozoa has been carried out because these can be used in artificial insemination or vitro fertilization. The purpose this study was to obtain information on the quality which produced from Bali cattle at different ages. samples came 31 slaughterhouses. Information and identification age based structure incisors. Observations were made variables motility, con...

The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 x 10(-3) M, 1 x 10(-4) M, 1 x 10(-5) M and 1 x 10(-6) M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved ...