Abstract Immobilized microbial technology has been widely used in wastewater treatment, but it less frequently for soil remediation, particularly sites that are co-contaminated with organic compounds and heavy metals. In addition, there is limited knowledge on the efficiency of remediation preferences to colonize immobilized carriers. this study, biochar Sphingobium abikonense was introduced re...

Lignin is the most abundant aromatic bioresource in nature, and elucidation of its biodegradation system essential for understanding carbon cycling on earth effective usage. The β−5 bond one intermolecular linkages lignin. Sphingobium sp. strain SYK-6 can assimilate various lignin-derived compounds, including a bond-containing dimer (phenylcoumaran-type), dehydrodiconiferyl alcohol (DCA). In ca...

Hexabromocyclododecanes (HBCDs) were used as flame-retardants until their ban in 2013. Among the 16 stereoisomers known, ε-HBCD has highest symmetry. This makes an interesting substrate to study selectivity of biotransformations. We expressed three LinA dehydrohalogenase enzymes E. coli bacteria, two wild-type, originating from Sphingobium indicum B90A bacteria and LinATM, a triple mutant LinA2...

Pentachlorophenol (PCP), a highly toxic anthropogenic pesticide, can be mineralized by Sphingobium chlorophenolicum, a gram-negative bacterium isolated from PCP-contaminated soil. However, degradation of PCP is slow and S. chlorophenolicum cannot tolerate high levels of PCP. We have used genome shuffling to improve the degradation of PCP by S. chlorophenolicum. We have obtained several strains ...

PcpR is a LysR-type transcription factor from Sphingobium chlorophenolicum L-1 that is responsible for the activation of several genes involved in polychlorophenol degradation. PcpR responds to several polychlorophenols in vivo. Here, we report the crystal structures of the inducer-binding domain of PcpR in the apo-form and binary complexes with pentachlorophenol (PCP) and 2,4,6-trichlorophenol...

Dichlorohydroquinone dioxygenase (PcpA) is the ring-cleavage enzyme in the PCP biodegradation pathway in Sphingobium chlorophenolicum strain ATCC 39723. PcpA dehalogenates and oxidizes 2,6-dichlorohydroquinone to form 2-chloromaleylacetate, which is subsequently converted to succinyl coenzyme A and acetyl coenzyme A via 3-oxoadipate. Previous studies have shown that PcpA is highly substrate-spe...