A pressing need for new chronic kidney disease (CKD) biomarkers persists. MicroRNAs (miRNAs) are emerging as a novel class of disease biomarkers in body fluids, but mechanisms conferring their stability in urine have not been fully elucidated. Here we investigated stabilization in human urine of ubiquitously expressed miR-16, and miR-192, which we have shown previously to be downregulated in re...

Human urine specimens that were determined to be presumptively positive for oxycodone and its metabolite, oxymorphone, by immunoassay screening were assayed using fast gas chromatography-mass spectrometry to positively identify and quantify the oxycodone and oxymorphone present. Urine specimens were first spiked with deuterated internal standards, oxycodone-d(3) and oxymorphone-d(3), subjected ...

A simple, accurate and sensitive voltammetric method for determination of Anagrelide (ANG) using β cyclodextrin modified carbon paste electrode (CDMCPE) is developed. CDMCPE exhibited significantly increased sensitivity and selectivity for ANG compared to Carbon paste electrode. Peak currents showed a linear response in the concentration range 1.8 × 10-9 to 1.6 × 10-6 M at CDMCPE. Limit of dete...

INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: The assay showed 100% specificity against 17 Leptospira strains with a limit ...

In this work, two approaches based on Dispersive Liquid-Liquid MicroExtraction (DLLME) and Dispersive Liquid-Liquid MicroExtraction based on Solidification of Floating Organic Drop (DLLME-SFO) were compared for the extraction and preconcentration of crocin from saffron and biological samples. Different DLLME and DLLME-SFO parameters influencing the extraction efficiency were studied and opt...

BACKGROUND Human exposure to bisphenol A (BPA) is ubiquitous, yet there are concerns about whether BPA can be measured in human blood. This Round Robin was designed to address this concern through three goals: 1) to identify collection materials, reagents and detection apparatuses that do not contribute BPA to serum; 2) to identify sensitive and precise methods to accurately measure unconjugate...

Cross-reactivities of some over-the-counter and uncontrolled prescription drugs with the immunoassays of some classes of drugs of abuse have been reported (1–17). Chemical structure similarity is a cause for cross-reactivity in immunoassays because the cross-reacting compound can fit onto the active sites in the antibody that has been developed for the drug of abuse or its chemical class. Howev...

A simple, selective, and sensitive method involving a miniaturized solid phase extraction step based on monolithic molecularly imprinted polymer (MIP) directly coupled on-line to UV detection was developed for the determination of benzoylecgonine (BZE) in complex biological samples. Monolithic MIPs were prepared into 100 μm internal diameter fused-silica capillaries either by thermal or photopo...