Infection of cultured insect cells with Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) resulted in the generation of mutants with major genomic deletions. Some of the mutants lacked the ability to infect S. exigua larvae per os. The gene(s) responsible for this phenotype in SeMNPV was mapped within a contiguous sequence encoding ORFs 29-35. In this paper we have shown that SeMNPV O...

The first-generation transgenic cotton (Gossypium hirsutum L.), Bollgard, which contains a single toxin protein gene cry1Ac from Bacillus thuringiensis var. kurstaki Berliner, was approved by the National Biosafety Committee (NBC) for commercial growing in Pakistan in 2011 and the yield enhancement is promising, with an increase in cotton bales up to 150 millionthe record productivity of the co...

Abstract Transgenic plants that express double-stranded RNA (dsRNA) targeting vital insect genes have recently emerged as a valuable new tool for pest control. In this study, tobacco were transformed to produce dsRNA Sl 102 gene is involved in the immune response of Spodoptera littoralis larvae, serious lepidopteran several crops. Experimental larvae reared on transgenic lines showed (1) strong...