Hepatitis C virus (HCV) populations in vivo consist of heterogeneous mixtures of genetically different but closely related variants defined as a 'quasispecies'. The longitudinal fluctuation of HCV quasispecies populations in chronic hepatitis C has not been elucidated. Serial plasma samples were obtained from four patients with chronic hepatitis C (two patients without any treatment and two pat...

genotypes of iranian zel sheep for calpastatin(cast) locus were determined by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) methods and for calpain(capn) locus by pcr-sscp. blood samples were collected from 200 purebred zel sheep of zel breeding station located in golestan province i...

In this study, Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance was analyzed and compared by three molecular methods such as DNA  sequencing, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR-single strand conformation polymorphism (PCR-SSCP) in 105 phenotypically resistant isolates obtained from various parts of Ind...

DOI: 10.1002/sscp.202200019 The cover picture shows that the lead ion (Pb2+) and copper (Cu2+) in lake water are detected by UV-Vis analysis using gold nanoparticles (Au NPs) as colorimetric probe. formation of Pb-O bond between Pb2+ L-Cysteine can induce significant aggregation Au NPs (blue color), conversely, Cu catalyze oxidation Cysteine prevent (pink color). By analyzing absorption spectra...

Single-strand conformation polymorphism (SSCP) analysis is a simple and quick method for mutation detection. It depends on the separation of single-stranded (ss)DNA fragments in non-denaturing polyacrylamide gels. On occasion, the ssDNA bands can be very diffuse or appear against a heavily smeared background. Here, we demonstrate that the addition of denaturants, urea or formamide, to the gels ...

the drb3 gene is a highly polymorphic major histocompatibility complex (mhc) class ii gene and plays an important role in variability of immune responsiveness and disease resistance. in the present study, the mhc class ii drb3 gene in water buffalo (bubalus bubalis) populations from northwest regions of iran was investigated through pcr-sscp. genomic dna was extracted from whole blood samples c...

Familial hypercholesterolaemia (FH) is an autosomal codominant disorder characterised by high levels of LDL cholesterol and a high incidence of coronary artery disease. Our aims were to track the low density lipoprotein receptor (LDLR) gene in individual families with phenotypic FH and to identify and characterise any mutations of the LDLR gene that may be common in the west of Scotland FH popu...