Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...

Background: Most cancer studies focus on exploring non-invasive biomarkers for cancer detection. In the present study, we sought to investigate the expression level of microRNA-21 (miR-21), as a potential diagnostic marker, in serum and stool samples from 40 patients with colorectal cancer (CRC) and 40 healthy controls. Methods: Quantitative real-time RT-PCR was applied to determine the relativ...

The hypothesis that a toxic hemolytic substance could be recovered from the stools of pernicious anemia patients was investigated. Saline stool extracts of patients suffering from pernicious anemia and those of normal individuals were administered to rabbits by intravenous and subcutaneous injection over long periods of time. Stool extracts prepared by the special technique of Seyderhelm (1) we...

background: strongyloidiasis is mostly an asymptomatic infection and diagnosis of latent infec­tions is difficult due to limitations of current parasitological and serological methods. this study was conducted to set up a pcr-based method for molecular diagnosis of strongyloides stercor­alis infection by detection of copro-dna in stool samples. methods: a total of 782 fresh stool samples were c...

Cryptosporidium spp. infection is one of the causes diarrhea in people living with HIV/AIDS. The objective this study to compare sensitivity microscopy and molecular biology determine prevalence Patients Living With HIV (PLWH). This a descriptive cross-sectional conducted three care centers for HIV/AIDS Abidjan. It took place from November 2018 March 2020. Sociodemographic data were obtained vi...

Rapid and effective methods for the isolation of Clostridium difficile from stool samples are desirable to obtain isolates for typing or to facilitate accurate diagnosis of C. difficile-associated diarrhea. We report on the evaluation of a prototype chromogenic medium (ID C. difficile prototype [IDCd]) for isolation of C. difficile. The chromogenic medium was compared using (i) 368 untreated st...

OBJECTIVE To evaluate the diagnostic performance of Xpert® MTB/RIF on stool samples from children with clinical suspicion of pulmonary tuberculosis (PTB) at primary care clinics. DESIGN A cross-sectional diagnostic evaluation enrolling 5-16 year olds from whom one induced sputum (IS) sample was tested for microbiological TB confirmation. Results of a single stool sample tested using Xpert wer...

This study was performed on 1350 school children from 9 different villages in Sharkia Governorate to investigate the real situation of endemicity of fascioliasis in the area. Stool examination using modified Kato thick smear method was performed to detect Fasciola infection and other parasites. Those with negative stool samples were examined serologically by ELISA test to detect anti-Fasciola I...