Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was...

It has recently been proposed by Wellington et al. that the genus Kitasatosporia Omura, Takahashi, Iwai and Tanaka (9) is a synonym of the genus Streptomyces, and four validly published species, Kitasatosporia setae (9), Kitasatosporia griseola (12), Kitasatosporia phosalacinea (12) and Kitasatosporia mediocidica (3), have been transferred to the genus Streptomyces as new combinations, Streptom...

the streptomycetes are gram positive bacteria with a filamentous form that present in a wide variety of soil including composts, water and plants. the most characteristic of streptomycetes is the ability to produce secondary metabolites such as antibiotics. they produce over two-thirds of the clinically useful antibiotics of natural origin (e.g., neomycin and chloramphenicol. another characteri...

produced by mutants of Streptomyces peucetius^ , the daunorubicin2) producing microorganism, led to the discovery of doxorubicin (adriamycin)3) and other new anthracyclines4~8). By further mutagenic treatment of Streptomyces peucetius var. aureus, sl mutant of Streptomyces peucetius producing 4Odemethyl1 1 deoxydoxorubicin and analogues7), we have recently isolated other new mutant strains. Amo...

Background and Purpose: Cutaneous infections arise from a homogeneous group of keratinophilic fungi, known as dermatophytes. Since these pathogenic dermatophytes are eukaryotes in nature, use of chemical antifungal agents for treatment may affect the host tissue cells. In this study, we aimed to evaluate the antifungal activity of Actinomyces species against Trichophyton mentagrophytes (abbrevi...

BACKGROUND The Actinomycete Actinosynnema pretiosum ssp. auranticum has commercial importance due to its production of ansamitocin P-3 (AP-3), a potent antitumor agent. One way to increase AP-3 production would be to constitutively express selected genes so as to relieve bottlenecks in the biosynthetic pathway; however, an integrative expression vector for A. pretiosum is lacking. The aim of th...

Protoplast fusion was investigated as a technique for genetically manipulating two lignin-degrading Streptomyces strains, Streptomyces viridosporus T7A and Streptomyces setonii 75Vi2. Four of 19 recombinants tested showed enhanced production of acid-precipitable polymeric lignin (APPL), producing 155 to 264% more APPL from corn stover lignocellulose than was produced by the wild-type S. viridos...

Mangroves are the tidal forest existing in the intertidal zone and usually considered as the special marine ecosystem. In the present study, 452 actinomycetes were recovered from nine diferent sites at Maowei Sea Mangrove Reserve in Qinzhou (Guangxi province, China). Among them, Seventy-four strains were purified for 16s RNA gene sequencing and further characterization. The results indicated th...

Mangroves are the tidal forest existing in the intertidal zone and usually considered as the special marine ecosystem. In the present study, 452 actinomycetes were recovered from nine diferent sites at Maowei Sea Mangrove Reserve in Qinzhou (Guangxi province, China). Among them, Seventy-four strains were purified for 16s RNA gene sequencing and further characterization. The results indicated th...

We present STEREO, a system that offers an expressive formalism and implements techniques firmly grounded on logic to solve the Service Selection Problem (SSP). STEREO adopts the Local-As-View approach (LAV) to represent services’ functionality as views on ontology concepts, while user requests are expressed as conjunctive queries on these concepts. Additionally, users can describe their prefer...