A method is described for the rapid preparation of peroxisomes from rat liver by using sucrose-density-gradient centrifugation in a vertical rotor. The preparation, shown to be virtually free of mitochondrial and microsomal contamination, can be used to study fatty acid metabolism by isolated peroxisomes.

A new species of ribonucleic acid (RNA) was detected in sporulating culture of Saccharomyces cerevisiae. This RNA was isolated by sucrose density gradient centrifugation and polyacrylamide gel electrophoresis and partially characterized. It has a sedimentation coefficient of approximately 20S and a nucleotide composition distinct from other known RNA species in yeast, and it hybridizes with nuc...

The puromycin-induced dissociation, at high ionic strength, of active ribosomes from reticulocytes resulted in the release of protein-free, apparently undegraded, messenger RNA for globin, which was identified by centrifugation on a sucrose density gradient. This procedure should make messenger RNA from various cells available for isolation on a large scale, and has several advantages over proc...

Sucrose density gradient ultracentrifugation can be used to detect specific 19S antibodies of the IgM class in the sera of patients recently infected with influenza A virus, provided steps are taken to remove non-specific inhibitors of haemagglutination. The usefulness of the procedure for the diagnosis of influenza requires further evaluation.

Various catenated and replicating molecules of colicin E1 isolated from minicells have been identified in regions of neutral sucrose density gradients or cesium chloride-ethidium bromide density gradients. These colicin molecules have been found in those regions of the gradient where pulse-labeled DNA accumulates.

A simple technique has been developed with which five serum specimens can be chromatographed at one time and concentrated immunoglobulin M fractions can be obtained without using fraction collectors and ultraviolet monitors. When applied to the detection of rubella immunoglobulin M antibody, this technique gave results comparable to the sucrose gradient centrifugation method.

Magnetic Nanoparticles Using Sucrose Density Gradient Ultracentrifugation Andrew M. Prantner,†,* Jun Chen,‡ Christopher B. Murray,‡,§ and Nathalie Scholler† †Penn Ovarian Cancer Research Center and Department of Obstetrics and Gynecology, University of Pennsylvania Perelman School of Medicine, ‡Department of Chemistry, and Department of Materials Science and Engineering, University of Pennsylva...

An immunoglobulin M response to varicella-zoster virus was detected in 70% of zoster patients by solid-phase radioimmunoassay, in 52% by indirect immunofluorescence, in 48% by neutralization on sucrose density gradient fractions, and in 27% by an antibody class capture enzyme immunoassay. The patients showed marked variations in their varicella-zoster virus immunoglobulin M responses detectable...

Total soluble solid (TSS) content is commercially used as fruit quality index because of its high positive correlation with sugar content. In the present study, The effect of foliar application of naphthalene acetic acid (NAA) with different concentrations (0, 25, 50, and 100 mg.L-1) at 4 true leaf and fruit set stages and plant training (pruning and thinning) on TSS and soluble sugar (i.e. suc...

Leaves of 10 plant species, 7 with photorespiration (spinach, sunflower, tobacco, pea, wheat, bean, and Swiss chard) and 3 without photorespiration (corn, sugarcane, and pigweed), were surveyed for peroxisomes. The distribution pattern for glycolate oxidase, glyoxylate reductase, catalase, and part of the malate dehydrogenase indicated that these enzymes exist together in this organelle. The pe...