The interaction of two isoenzymes of lactate dehydrogenase from pig heart muscle (H(4)) and rabbit skeletal muscle (M(4)), with immobilized nucleotides was examined: the effects of pH and temperature on the binding of lactate dehydrogenase were studied with immobilized NAD(+) matrices. The influence of substrate, product and sulphite on the binding of heart muscle lactate dehydrogenase to immob...

Wilson and Blair (1927) (1931) described a nutrient glucose bismuth-sulphite and iron medium useful in detecting the presence of typhoid-paratyphoid organisms in water and sewage. The method was modified by Thompson (1939) for clinical use in the identification of clostridia. A positive test is evidenced by diffuse or localized blackening of the medium around sites of bacterial growth. Heavily ...