Highly purified iron superoxide dismutase was obtained from Escherichia coli B using a modification of the procedure of Yost and Jridovich (Yost, F. J., Jr., and Fridovich, I. (1973) J. Biol. Chem. 248, 4905-4908). The protein contained 1.8 +/- 0.2 atoms of iron per 38,700 g of protein. We have found that cyanide does not bind to the Fe3+ ion of iron dismutase but fluoride and azide have modera...

Fifty-two ovine strains of Pasteurella haemolytica and P. trehalosi representing serotypes 1-16 were examined for the presence of [copper, zinc]superoxide dismutase DNA sequences. This was done using a combination of polymerase chain reaction with degenerate primers based on the sequence of the [Cu,Zn]superoxide dismutase gene (sodC) in related species and Southern hybridization using a fragmen...

The major superoxide dismutase of the unicellular red alga, Porphyridium cruentum, has been purified to homogeneity. This enzyme has a molecular weight of 40,000 and is composed of two subunits of equal size, which are joined by noncovalent interactions. Manganese constituted 0.13% of this superoxide dismutase. T,is is equivalent to 1 manganese atom/molecule of enzyme. Cyanide at 5 mM and H2O2 ...

background: the aim of this study was to compare superoxide dismutase (sod) activity in fasciola hepatica and fasciola gigantica parasites. materials: f. gigantica and f. hepatica helminths were collected from abattoir and cultured in buffer media for 4 h at 37 °c. excretory-secretory (es) products were collected, centrifuged and stored at - 20 ◦ c. e-s protein concentration was measured by bra...

conclusions consumption of ole after tumor transplantation reduced the tumor growth. on the other hand, owing to its highly-packed polyphenols, catalase and superoxide dismutase activities in tumor tissue were increased. results treatment with 150 and 225 mg/kg/day of ole reduced the tumor volume and weight (p < 0.05) compared to the first group significantly. it also increased the cat activity...

patients and methods manganese superoxide dismutase polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) in 103 patients with mlds and 103 healthy control subjects. conclusions according to the fact that mn-sod gene polymorphisms have been considered as a major risk factor in some malignancies, this single center study did not find any a...

the aims of this study were to assess the effects of drought stress on peroxidase (pod), superoxide dismutase (sod) and catalase (cat) activities as well as free proline content and growth parameters of pistacia khinjuk seedlings under drought stress. therefore, the one-year seedlings of pistacia khinjuk subjected to water stress (25%, 50%, 75%, and 100% field capacity) for 8 months in greenhou...

cadmium is considered as a common residue in water and sediments which could readily enter aquatic organisms. the aim of this study was to evaluate the time- and concentration-dependent changes in antioxidant enzymes (sod and cat) activities as well as concentration of mda as a bi- product of lipid peroxidation in the liver of persian sturgeon, acipenser persicus , following cdcl 2 exposure at ...

introduction: considering the prevalence of epilepsy and the failure of available treatments for many epileptic patients, finding more effective drugs in the treatment of epilepsy seems necessary. oxidative stress has a special role in the pathogenesis of epileptic syndrome. therefore, in the present study, we have examined the anti-epileptic and anti-oxidant properties of the ferula assa foeti...

INTRODUCTION Preeclampsia is a complex syndrome of unknown aetiologic origin. It is characterized by the clinical triad of hypertension, edema and protenuria. Current concepts of the genesis of preeclampsia include endothelial dysfunction and oxidative stress. OBJECTIVE This study assessed the relationship between the activity of superoxide dismutase, the zincemia and the preeclampsia. METH...